Micropumps actuated negative pressure injection for microchip electrophoresis

Electrophoresis
Bowei LiBingcheng Lin

Abstract

A simple negative pressure pinched sample injection method was presented. This method combined diaphragm micropumps and a single voltage supply to generate controllable well-defined sample plug, and led to effective electrophoresis separation. The pinched plug was found to be favorable for obtaining representative and reproducible results that the RSD of the migration time and peak height of sodium fluorescein were 0.5 and 2.1%, respectively (n=25). The established method had been applied in separation of amino acid samples. This method has the advantages of well-defined plug, free sample bias effect, high reproducibility and convenience of controlling the negative pressure by the integrated pumps on the microchip. In addition, the single high voltage supply and the world-to-chip interface simplified the instrumentation, which is of benefit to the minimization and automation. These advantages demonstrate the potential of this method for a wide range of applications.

References

Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brian M PaegelRichard A Mathies
Jul 2, 2002·Analytical Chemistry·Darwin R ReyesAndreas Manz
Jul 2, 2002·Analytical Chemistry·Pierre-Alain AurouxAndreas Manz
Nov 13, 2003·Electrophoresis·Niels LionHubert H Girault
Apr 22, 2004·Lab on a Chip·Xiaoxia BaiHubert H Girault
Sep 7, 2004·Electrophoresis·Xiaomian ZhouBingcheng Lin
Dec 2, 2004·Analytical Chemistry·Xiuli MaoBingcheng Lin
Jun 1, 2005·Analytical Chemistry·James M KarlinseyJames P Landers
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Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christopher J EasleyJames P Landers
Feb 3, 2007·Lab on a Chip·Youlan LiKaran V I S Kaler
Nov 22, 2007·Lab on a Chip·Nannan YeBingcheng Lin

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Citations

Feb 26, 2013·Analytical and Bioanalytical Chemistry·Gaozhi OuBi-Feng Liu
Apr 17, 2012·Analytica Chimica Acta·James M Karlinsey

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