Micropuncture determination of pH, PCO2, and total CO2 concentration in accessible structures of the rat renal cortex

The Journal of Clinical Investigation
T D DuBoseN W CARTER

Abstract

Previous studies evaluating the mechanism of renal HCO-3 reabsorption have assumed equilibrium between systemic arterial blood and tubular fluid PCO2. We have recently reported that the PCO2 in proximal and distal tubular fluid as well as the stellate vessel significantly exceeded arterial PCO2 by 25.9 +/- 0.92 mm Hg. The purpose of this study was to determine directly, for the first time, pH, PCO1, and total CO2 concentration in the accessible structures of the rat renal cortex with both microelectrodes and microcalorimetry. In addition, the concentrations of chloride and total CO2 were compared in the stellate vessel. The data demonstrate that: (a) values for total [CO2] in both the proximal tubule and stellate vessel calculated from in situ determination of pH and PCO2 closely agree with the measured values for total [CO2]: (b) values for chloride concentration in the stellate vessel are significantly less than the corresponding values in systemic plasma (delta[Cl-] = 5.6 meq/liter); and (c) the rise in [HCO-3] from systemic to stellate vessel plasma closely approximates the observed reciprocal fall in [Cl-] in this structure.

References

May 1, 1990·Pflügers Archiv : European journal of physiology·M BidetP Poujeol
Aug 1, 1980·Pflügers Archiv : European journal of physiology·F J GennariC R Caflisch
Oct 1, 1985·Pflügers Archiv : European journal of physiology·A Brisolla-DiuanaG Malnic
Dec 1, 1993·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·M Baum, R Quigley
Oct 21, 2009·Pflügers Archiv : European journal of physiology·Ricardo Villa-Bellosta, Víctor Sorribas
Sep 21, 2012·American Journal of Physiology. Renal Physiology·Ryan J CorneliusSteven C Sansom
Jan 1, 1983·The Journal of Clinical Investigation·T D DuBose, M S Lucci
May 1, 1983·The Journal of Clinical Investigation·R M Effros, S Nioka
Oct 1, 1983·The Journal of Clinical Investigation·D A Maddox, F J Gennari
Apr 1, 1984·The Journal of Clinical Investigation·J P FrommerN A Kurtzman
Dec 1, 1986·The Journal of Clinical Investigation·G CapassoG Giebisch
Jul 1, 1987·The Journal of Clinical Investigation·E Pastoriza-MunozM L Graber
Aug 1, 1987·The Journal of Clinical Investigation·G CapassoG Malnic
Jun 1, 1989·The Journal of Clinical Investigation·P A Preisig, R J Alpern
Mar 21, 1998·The Journal of Clinical Investigation·D E WessonD F Green
Aug 28, 2010·Journal of the American Society of Nephrology : JASN·Xuming SunSnezana Petrovic
Mar 5, 2011·Journal of the American Society of Nephrology : JASN·Tomokazu SoumaSadayoshi Ito
May 16, 2014·BioMed Research International·W Patrick Burgess, Phillip J Walker
Dec 1, 1980·Pflügers Archiv : European journal of physiology·B CormanC de Rouffignac
Aug 1, 1987·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·R M Richardson, Mitchell L Halperin
Nov 1, 1982·Kidney International·C A Berry, D G Warnock
Mar 1, 1982·Kidney International·D BatlleN A Kurtzman
Jun 1, 1994·Kidney International·G CapassoG Giebisch
Dec 1, 1995·Kidney International·M Baum, R Quigley
Aug 19, 2015·PloS One·Lorette NoiretS Randall Thomas
Nov 12, 2016·Frontiers in Physiology·Ufuk Olgac, Vartan Kurtcuoglu
Aug 5, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jennifer P NgoRoger G Evans
Oct 1, 1984·Circulation Research·R M EffrosP Silverman
Jan 1, 1984·Annals of the New York Academy of Sciences·R M EffrosK Taki
Sep 1, 2005·American Journal of Physiology. Renal Physiology·Don-On Daniel MakConnie M Westhoff
Aug 21, 2007·Clinical Journal of the American Society of Nephrology : CJASN·Mitchell L HalperinKamel S Kamel
Jan 31, 2020·Physiological Reviews·Jens Leipziger, Helle Praetorius
Feb 1, 1996·Pediatric Research·J N SheuR Quigley

Citations

Feb 1, 1976·Kidney International·G Malnic, P R Steinmetz
Jun 22, 1976·Pflügers Archiv : European journal of physiology·E M Filho, G Malnic
Jun 1, 1976·Analytical Biochemistry·L R Pucacco, N W CARTER
Aug 1, 1978·Kidney International·C R CaflischN W CARTER
Aug 1, 1978·The Journal of Clinical Investigation·T D DuBoseN W CARTER
Apr 1, 1975·Analytical Chemistry·G G VurekR Corsey
Jan 1, 1974·Pflügers Archiv : European journal of physiology·E Frömter, K Gessner
Jun 1, 1974·Acta Physiologica Scandinavica·B Karlmark, B G Danielson
Jul 1, 1956·The American Journal of the Medical Sciences·E H BRESLER
Feb 1, 1965·The Journal of Clinical Investigation·F C RECTORD W SELDIN

Related Concepts

Chloride Ion Level
Microelectrodes
Congenital Chloride Diarrhea
Plasma Cell Neoplasm
Distal Convoluted Renal Tubule Structure
Carbonic Acid Ions
Calorimetry
Structure of Cortex of Kidney
Carbon Dioxide
Kidney Tubules, Proximal

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