Microreactor array device

Scientific Reports
Peter WiktorJoshua LaBaer

Abstract

We report a device to fill an array of small chemical reaction chambers (microreactors) with reagent and then seal them using pressurized viscous liquid acting through a flexible membrane. The device enables multiple, independent chemical reactions involving free floating intermediate molecules without interference from neighboring reactions or external environments. The device is validated by protein expressed in situ directly from DNA in a microarray of ~10,000 spots with no diffusion during three hours incubation. Using the device to probe for an autoantibody cancer biomarker in blood serum sample gave five times higher signal to background ratio compared to standard protein microarray expressed on a flat microscope slide. Physical design principles to effectively fill the array of microreactors with reagent and experimental results of alternate methods for sealing the microreactors are presented.

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Citations

Mar 12, 2015·Proteomics·Xiaofang BianJoshua LaBaer
Jan 11, 2017·ACS Applied Materials & Interfaces·Wei WangShaoliang Lin
Feb 23, 2017·Molecular & Cellular Proteomics : MCP·Lusheng SongJacqueline M Achkar
Apr 21, 2018·Sensors·Phenix-Lan QuanEric Brouzes
Jun 28, 2019·Frontiers in Cellular and Infection Microbiology·Kathryn J PflughoeftDavid P AuCoin
Feb 8, 2019·Chembiochem : a European Journal of Chemical Biology·Normann KilbGünter Roth
Apr 5, 2018·Nano Letters·Divya BalakrishnanCésar Pascual-García

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Methods Mentioned

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enzyme-linked immunosorbent assay

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