MicroRNA-101 (miR-101) post-transcriptionally regulates the expression of EP4 receptor in colon cancers.

Cancer Biology & Therapy
Anupama ChandramouliMark A Nelson

Abstract

Expression of the PGE2 receptor, EP4, is up-regulated during colorectal carcinogenesis. However the mechanism leading to deregulation of the EP4 receptor is not known. The present study was conducted to investigate the regulation of EP4 receptor by miRNAs. We analyzed 26 colon cancers (i.e. 15 adenocarcinomas and 9 adenomas) and 16 normal colon specimens for EP4 receptor expression by immunohistochemistry. A bioinformatics approached identified putative microRNA binding sites with the 3'-UTR of the EP4 receptor. Both colon cancer cell lines and tumor specimens were analyzed for miR-101 and EP4 expression by qRT-PCR and Western analysis respectively and simultaneously in situ hybridizations was used to confirm our results. In vitro and in vivo assays were used to confirm our clinical findings. We observed an inverse correlation between the levels of miR-101 and EP4 receptor protein. Transfection of LS174T cells with miR-101 significantly suppressed a luciferase reporter containing the EP4 receptor-3'-UTR. In contrast, a mutant EP4 receptor-3'-UTR construct was unaffected. Ectopic expression of miR-101 markedly reduced cell proliferation and motility. Co-transfection of EP4 receptor could rescue colon cancer cells from the tumor ...Continue Reading

References

Feb 21, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P RougierM Ducreux
Nov 23, 2000·The New England Journal of Medicine·C BombardierUNKNOWN VIGOR Study Group
Mar 30, 2001·The Journal of Biological Chemistry·H ShengR N DuBois
Mar 4, 2003·Nature Reviews. Drug Discovery·Rod J Flower
Mar 12, 2004·Genes & Development·John G Doench, Phillip A Sharp
May 5, 2004·The Journal of Biological Chemistry·Ambra PozziJorge H Capdevila
Jul 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Feb 4, 2005·The New England Journal of Medicine·Jeffrey A Meyerhardt, Robert J Mayer
Feb 17, 2005·The New England Journal of Medicine·Scott D SolomonUNKNOWN Adenoma Prevention with Celecoxib (APC) Study Investigators
Apr 20, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Joanne R Brown, Raymond N DuBois
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Nov 15, 2006·Annual Review of Medicine·Yong I Cha, Raymond N DuBois
Feb 10, 2007·International Journal of Cancer. Journal International Du Cancer·Annika GustafssonKent Lundholm
Jul 21, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Vincenza LeoneAngela M Acquaviva
Sep 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Anindita ChakrabartySudhansu K Dey
Oct 6, 2007·Molecular Carcinogenesis·Adriana DoldanJiaqi Shi
Nov 22, 2007·Journal of Cardiovascular Pharmacology·Colin D Funk, Garret A FitzGerald
Nov 14, 2008·Histochemistry and Cell Biology·Elena I Deryugina, James P Quigley
Nov 15, 2008·Science·Sooryanarayana VaramballyArul M Chinnaiyan
Jan 10, 2009·Experimental Cell Research·Antonio StrillacciVittorio Tomasi
Aug 8, 2009·The Journal of Clinical Investigation·Silvia Alvarez-DíazAlberto Muñoz
Feb 20, 2010·Nature Reviews. Cancer·Dingzhi Wang, Raymond N Dubois
Mar 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nicola ValeriCarlo M Croce
May 18, 2010·The Journal of Organic Chemistry·Danny GauvreauIan W Davies
Jan 11, 2011·Bioorganic & Medicinal Chemistry Letters·Jason D BurchYongxin Han

❮ Previous
Next ❯

Citations

Sep 22, 2012·Seminars in Immunopathology·Masako Nakanishi, Daniel W Rosenberg
Apr 26, 2014·World Journal of Gastroenterology : WJG·Jing-Jia Ye, Jiang Cao
Sep 18, 2013·Cancer Genetics·Jennifer HutchisonMark A Nelson
Dec 7, 2014·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Song LinZeng-Wu Shao
Jun 13, 2015·International Journal of Molecular Sciences·Martyna O UrbanekWlodzimierz J Krzyzosiak
Jul 21, 2015·FEBS Letters·Melania E Mercado-PimentelMark A Nelson
Jan 27, 2015·Genes, Chromosomes & Cancer·Raheleh AmirkhahAli Farazmand
May 23, 2013·Experimental Cell Research·Benjamin Chidi OnyeaguchaMark A Nelson
Jun 10, 2016·Journal of Human Genetics·Yoshiaki KitaShoji Natsugoe
Feb 18, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Chen LinGulinaer Kuerban
Dec 3, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Fei HuangChen Lin
Sep 18, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Ayla Valinezhad Orang, Abolfazl Barzegari
Aug 5, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ivy Ka Man LawCharalabos Pothoulakis
Nov 12, 2015·World Journal of Gastroenterology : WJG·Marco RagusaMichele Purrello
Dec 12, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Qifeng WangXiang Du
Nov 20, 2020·Revista Española De Enfermedades Digestivas : Organo Oficial De La Sociedad Española De Patología Digestiva·Zhuoran HuangJun Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.