MicroRNA-106b is involved in transforming growth factor β1-induced cell migration by targeting disabled homolog 2 in cervical carcinoma

Journal of Experimental & Clinical Cancer Research : CR
Yuan ChengLi Geng

Abstract

MicroRNA-106b (miR-106b) was recently identified as an oncogene participating in cancer progression. Transforming growth factor β1(TGF-β1) is an indispensable cytokine regulating the local microenvironment, thereby promoting cervical cancer progression. However, the roles of miR-106b in cervical carcinoma progression and TGF-β1-involvement in the tumorigenesis of cervical cancer remain unknown. The expression of miR-106b in human cervical specimens was detected by real-time PCR analysis and in situ hybridization assay. The effect of miR-106b on cell migration was analyzed by scratch and transwell assays. TGF-β1 was used to induce cell migration. The expression of the miR-106b target gene DAB2 in human cervical tissues and cell lines were measured by qRT-PCR, western blot and immunohistochemistry. Dual-luciferase reporter assay was used to identify DAB2 as a miR-106b-directed target gene. miR-106b was frequently up-regulated in human cervical carcinoma specimens and cervical cancer cell lines. Over-expression of miR-106b significantly promoted HeLa and SiHa cells migration. Likewise, inhibition of miR-106b decreased HeLa and SiHa cells migration. The multifunctional cytokine TGF-β facilitates metastasis in cervical carcinoma. mi...Continue Reading

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Citations

May 7, 2016·Journal of Experimental & Clinical Cancer Research : CR·Piwen WangJaydutt V Vadgama
Apr 28, 2017·International Journal of Molecular Sciences·Kangyun LanHui Wang
Jan 5, 2019·Archives of Gynecology and Obstetrics·Shan ZongYing Yue
Feb 22, 2017·International Journal of Molecular Sciences·Angelica Judith Granados-LópezJesús Adrián López
Oct 3, 2018·Cell Death Discovery·Yuexiong YiWei Zhang
Mar 14, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Zhiming ZhengTao Song
Mar 21, 2019·Journal of Cellular Physiology·Javid Sadri NahandHamed Mirzaei
Jun 6, 2018·Cancer Biomarkers : Section a of Disease Markers·Yanying ZhaoKongxi Zhu
Apr 4, 2021·Cancers·Shailendra Kumar Dhar DwivediResham Bhattacharya
Mar 30, 2021·Frontiers in Oncology·Tanchun Cheng, Shouguo Huang
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dawid MehlichPaweł K Włodarski
May 5, 2021·Neoplasia : an International Journal for Oncology Research·Noreen McBreartyMark Feitelson
Jun 3, 2021·Biomolecules·Jaroslav NunvarRuth Tachezy

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Methods Mentioned

BETA
transfection
biopsy
PCR
Assay

Software Mentioned

Image J
GraphPad Prism
Pro Plus
Image
TargetScan

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