microRNA-106b-mediated down-regulation of C1orf24 expression induces apoptosis and suppresses invasion of thyroid cancer.

Oncotarget
Gianna CarvalheiraJanete Maria Cerutti

Abstract

We previously showed that C1orf24 expression is increased in thyroid carcinomas. Nonetheless, the mechanism underlying C1orf24 deregulation is not fully understood. It has been widely demonstrated that microRNAs are involved in post-transcriptional gene regulation in several diseases, including cancer. Using in silico prediction approach, five microRNAs that bind to the 3'-untranslated region (3'-UTR) of C1orf24 were identified. The expression of two selected microRNAs (miR-17-5p, miR-106b) and the expression of C1orf24 were tested in 48 benign and malignant thyroid lesions and in five thyroid carcinoma cell lines. miR-106b was down-regulated in thyroid cancer specimens and thyroid carcinoma cell lines, while C1orf24 expression was markedly increased. To demonstrate that miR-106b reduces C1orf24 expression, follicular (WRO) and papillary (TPC1) thyroid carcinoma cell lines were transiently transfected with miR-106b mimic. Ectopic expression of the miR-106b mimic significantly inhibits C1orf24 mRNA and protein expression in both WRO and TPC1 cells. Dual-luciferase report assays demonstrated that miR-106b directly targets C1orf24 by binding its 3'-UTR. Moreover, miR-106b-mediated down-regulation of C1orf24 expression increased ap...Continue Reading

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Citations

Mar 9, 2017·Journal of Experimental & Clinical Cancer Research : CR·Jing SunChuan Su
Nov 16, 2019·Pflügers Archiv : European journal of physiology·Kai-Kai ZhangXian-Quan Liang
Feb 6, 2020·Scientific Reports·Luka BrčićVesna Boraska Perica
Nov 8, 2018·Endocrine-related Cancer·Bruno Heidi NozimaJanete Maria Cerutti

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Methods Mentioned

BETA
PCR
transfection
surgical resection
Assay
scraping
PCA

Software Mentioned

ImageQuant
TargetScan
miRanda
PrimerX
Primer3
PicTar
CorelDraw Graphics Suite
GraphPad PRISM

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