MicroRNA-1205, encoded on chromosome 8q24, targets EGLN3 to induce cell growth and contributes to risk of castration-resistant prostate cancer

Oncogene
Yicun WangRunhua Liu

Abstract

The chromosome 8q24.21 locus, which contains the proto-oncogene c-MYC, long non-coding RNA PVT1, and microRNAs (miRs), is the most commonly amplified region in human prostate cancer. A long-range interaction of genetic variants with c-MYC or long non-coding PVT1 at this locus contributes to the genetic risk of prostate cancer. At this locus is a cluster of genes for six miRs (miR-1204, -1205, -1206, -1207-3p, -1207-5p, and -1208), but their functional role remains elusive. Here the copy numbers and expression levels of miRs-1204-1208 were investigated using quantitative PCR for prostate cancer cell lines and primary tumors. The data revealed that copy numbers and expression of miR-1205 were increased in both castration-resistant prostate cancer cell lines and in primary tumors. In castration-resistant prostate cancer specimens, the copy number at the miR-1205 locus correlated with the expression of miR-1205. Furthermore, functional analysis with an miR-1205 mimic, an miR-1205 inhibitor, and CRISPR/Cas9 knockout revealed that, in human prostate cancer cells, miR-1205 promoted cell proliferation and cell cycle progression and inhibited hydrogen peroxide-induced apoptosis. In these cells, miR-1205 downregulated the expression of t...Continue Reading

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Citations

Aug 6, 2019·Frontiers in Oncology·Wenxi WangZhaoyang Zeng
Jul 25, 2019·International Journal of Molecular Sciences·Eun-Ae KimTae-Jin Lee
Jun 30, 2019·Frontiers in Oncology·Camille DerderianOlorunseun O Ogunwobi
Nov 30, 2019·Frontiers in Oncology·Ariadna BoloixMiguel F Segura
Oct 13, 2020·Current Opinion in Endocrine and Metabolic Research·Morgan L ZennerLarisa Nonn
Apr 4, 2021·International Journal of Molecular Sciences·Niki M ZachariasNeema Navai
Sep 13, 2020·Pathology, Research and Practice·Cunjin NanYinghe Chen
Jul 3, 2021·International Journal of Molecular Sciences·Marwa MatboliSanaa Eissa
Aug 14, 2021·Frontiers in Cell and Developmental Biology·Michelle NaidooOlorunseun O Ogunwobi

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Methods Mentioned

BETA
PCR
laser capture microdissection
transfection
laser-capture
flow
xenograft
immunoprecipitation
ubiquitination
Assay
flow cytometry

Software Mentioned

StatView
Partek Genomic Suite ( PGS
micro
Excel
TargetScan
Diana
SPSS
microT

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