MicroRNA-1231 exerts a tumor suppressor role through regulating the EGFR/PI3K/AKT axis in glioma

Journal of Neuro-oncology
Jiale ZhangXiaoming Lu

Abstract

MicroRNAs (miRNAs) have been shown to be involved in the initiation and progression of glioma. However, the underlying molecular mechanisms are still unclear. We performed microarray analysis to evaluate miRNA expression levels in 158 glioma tissue samples, and examined miR-1231 levels in glioma samples and healthy brain tissues using qRT-PCR. In vitro analyses were performed using miR-1231 mimics, inhibitors, and siRNA targeting EGFR. We used flow cytometry, CCK-8 assays, and colony formation assays to examine glioma proliferation and cell cycle analysis. A dual luciferase reporter assay was performed to examine miR-1231 regulation of EGFR, and the effect of upregulated miR-1231 was investigated in a subcutaneous GBM model. We found that miR-1231 expression was decreased in human glioma tissues and negatively correlated with EGFR levels. Moreover, the downregulation of miR-1231 negatively correlated with the clinical stage of human glioma patients. miR-1231 overexpression dramatically downregulated glioma cell proliferation, and suppressed tumor growth in a nude mouse model. Bioinformatics prediction and a luciferase assay confirmed EGFR as a direct target of miR-1231. EGFR overexpression abrogated the suppressive effect of mi...Continue Reading

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Sep 26, 2020·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Seokhwi KimDakeun Lee
Feb 25, 2020·Journal of Oncology·Adrianna RutkowskaPiotr Rieske
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Dec 11, 2019·Oncology Research and Treatment·Yitao WangDan Zhao

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Datasets Mentioned

BETA
GSE10611

Methods Mentioned

BETA
surgical resection
Transfection
immunoprecipitation assay
electrophoresis
flow cytometry
Assay
xenograft
Fluorescence
antisense oligonucleotides
xenografts

Software Mentioned

ImageJ
GraphPad Prism
Multiplexer Viewer
miRGen
miRanda
TargetScan

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