Abstract
Lung cancer is a malignant tumor leading to the most cancer-related deaths worldwide. The treatment efficiency of lung cancer remains poor mainly due to chemotherapy drug resistance, including cisplatin. MicroRNAs (miRNAs) are closely related to chemotherapy resistance of tumor cells. Here, we illustrated the underlying mechanism of miR-1236-3p on the DDP resistance in lung cancer cells. In this study, we found that the expression level of miR-1236-3p was significantly decreased in lung cancer tissues and A549 cell line. In addition, the half maximal inhibitory concentration (IC50) of DDP in A549 cells was significantly lower than that in A549/DDP cells, while the expression level of miR-1236-3p was prominently down-regulated in A549/DDP cells. Combining the online tool TargetScan and a dual-luciferase reporter assay, tumor protein, translationally-controlled 1 (TPT1) was proved to be the direct target gene of miR-1236-3p. The MTT and flow cytometry assays demonstrated that up-regulation of miR-1236-3p could markedly inhibit A549/DDP cell proliferation but promote apoptosis, which could be significantly reversed by pcDNA3.1-TPT1 plasmids. Finally, we further demonstrated that miR-1235-3p could restrain the expression levels of ...Continue Reading
References
Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Dec 6, 2001·Nature Reviews. Genetics·S R Eddy
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Nov 13, 2004·International Journal of Cancer. Journal International Du Cancer·Chifumi FujiiNaofumi Mukaida
Mar 12, 2005·CA: a Cancer Journal for Clinicians·D Max ParkinPaola Pisani
Jul 5, 2006·Cancer Research·Ying-Yi LiNaofumi Mukaida
May 2, 2008·International Journal of Cancer. Journal International Du Cancer·Lin XiaDaiming Fan
Jul 23, 2008·Molecular Cancer Therapeutics·Olga KovalchukIgor P Pogribny
Nov 22, 2008·Respiration; International Review of Thoracic Diseases·Marco CiottiGiorgio Federici
Apr 11, 2009·International Journal of Oncology·Munirathinam GnanasekarKalyanasundaram Ramaswamy
Jul 23, 2009·Journal of Proteome Research·Qiang MaMing Li
Oct 6, 2011·Oncology Reports·Yong ZhangYun-Jie Wang
Jul 17, 2013·International Journal of Molecular Medicine·Tianzhu QiuPing Liu
Oct 30, 2013·Molecular Cancer Research : MCR·Fei ZhangYing-Yi Li
Feb 28, 2014·Oncology Reports·Yu WangBeihua Kong
Sep 13, 2014·Cell Death and Differentiation·K S R SastryL Chouchane
May 23, 2015·Scientific Reports·Yun LiuJifeng Feng
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Feb 11, 2016·Molecular Medicine Reports·Jin ZhangJun Wang
Feb 13, 2016·Oncology Letters·Wenfeng HuangChangjie Huang
Jun 21, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chuanchang LiZhong Chen
Jun 28, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yasutoshi TakashimaAkihiko Ito
Jul 12, 2017·Oncology Letters·Hong-Yan ZhangJi-Bin Lu
Aug 27, 2017·Biochemical and Biophysical Research Communications·Tingting BianJianguo Zhang
Sep 21, 2017·Journal of Cancer·Yu YouPing Bie
May 11, 2018·Cancer Cell International·Jia-Xiang AnDong-Qiu Dai
Citations
Dec 25, 2019·Scientific Reports·Peng ZouJi Yi
May 21, 2020·OncoTargets and Therapy·Haifeng WangZhongjie Chen
May 6, 2020·Cancer Management and Research·Rui Jia, Changli Wang
Sep 10, 2020·Molecular Medicine Reports·Guanghong ZhangJihong Luo
Dec 7, 2020·Cellular Signalling·Milad AshrafizadehHaroon Khan
Feb 9, 2021·3 Biotech·Chaofeng TangBendong Chen
Jan 16, 2021·Bioorganic Chemistry·Sarah Al-RashedMohd Sajid Khan
Dec 18, 2020·The Journal of Surgical Research·Jie HaoQinying Shi
Feb 20, 2020·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Naizhi WangQin Liu
Oct 19, 2021·Experimental and Therapeutic Medicine·Yuan Yuan ChengFang Min Xie