microRNA-1236-3p Regulates DDP Resistance in Lung Cancer Cells

Open Medicine
Zhigang WangWenxia Wang

Abstract

Lung cancer is a malignant tumor leading to the most cancer-related deaths worldwide. The treatment efficiency of lung cancer remains poor mainly due to chemotherapy drug resistance, including cisplatin. MicroRNAs (miRNAs) are closely related to chemotherapy resistance of tumor cells. Here, we illustrated the underlying mechanism of miR-1236-3p on the DDP resistance in lung cancer cells. In this study, we found that the expression level of miR-1236-3p was significantly decreased in lung cancer tissues and A549 cell line. In addition, the half maximal inhibitory concentration (IC50) of DDP in A549 cells was significantly lower than that in A549/DDP cells, while the expression level of miR-1236-3p was prominently down-regulated in A549/DDP cells. Combining the online tool TargetScan and a dual-luciferase reporter assay, tumor protein, translationally-controlled 1 (TPT1) was proved to be the direct target gene of miR-1236-3p. The MTT and flow cytometry assays demonstrated that up-regulation of miR-1236-3p could markedly inhibit A549/DDP cell proliferation but promote apoptosis, which could be significantly reversed by pcDNA3.1-TPT1 plasmids. Finally, we further demonstrated that miR-1235-3p could restrain the expression levels of ...Continue Reading

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Citations

Jan 16, 2021·Bioorganic Chemistry·Sarah Al-RashedMohd Sajid Khan
Feb 20, 2020·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Naizhi WangQin Liu
Oct 19, 2021·Experimental and Therapeutic Medicine·Yuan Yuan ChengFang Min Xie

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Methods Mentioned

BETA
surgical resection
transfection
PCR
flow cytometry
protein assay
X-ray

Software Mentioned

TargetScan
SPSS

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