MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivo

PloS One
C SaraivaKarsten Ruscher

Abstract

There is a high quest for novel therapeutic strategies to enhance recovery after stroke. MicroRNA-124 (miR-124) has been described as neuroprotective and anti-inflammatory molecule. Moreover, miR-124 is a well described enhancer of adult neurogenesis that could offer potentially beneficial effects. Herein, we used miR-124-loaded nanoparticles (miR-124 NPs) to evaluate their therapeutic potential in an in vitro and in vivo model of stroke. For that, neuroprotective and neurogenic responses were assessed in an in vitro model of stroke. Here, we found that miR-124 NPs decreased cell death and improved neuronal differentiation of subventricular zone (SVZ) neural stem cell cultures after oxygen and glucose deprivation. In contrast, intravenous injection of miR-124 NPs immediately after permanent focal ischemia induced by photothrombosis (PT) did not provide a better neurological outcome. In addition, treatment did not affect the number of 5-bromo-2'-deoxyuridine (BrdU)- and doublecortin/BrdU- positive cells in the SVZ at the study endpoint of 14 days after PT. Likewise, the ischemic insult did not affect the numbers of neuronal progenitors in the SVZ. However, in PT mice miR-124 NPs were able to specifically augment interleukin-6 le...Continue Reading

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Citations

Nov 22, 2019·Cellular and Molecular Life Sciences : CMLS·Ivo F MachadoAnabela P Rolo
Dec 28, 2019·International Journal of Molecular Sciences·Xiaolu LiuZhigang Mei
Dec 23, 2019·Acta Neuropathologica Communications·Daniela TalhadaKarsten Ruscher
Apr 7, 2019·Molecular Neurobiology·Murali VijayanP Hemachandra Reddy
Sep 2, 2020·International Journal of Molecular Sciences·Xianshuang LiuZhenggang Zhang
Jan 27, 2021·Drug Discovery Today·Amit Kumar SinghShripad A Patil
Oct 18, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sharon Wei Ling LeeValeria Chiono
Apr 23, 2021·Cellular and Molecular Neurobiology·Jinying XuGuangfan Chi
May 1, 2021·International Journal of Molecular Sciences·Amelia Eva AránegaFrancisco Hernández-Torres
Jun 3, 2021·Life·Sara Bernardo-CastroJoão Sargento-Freitas
Aug 21, 2021·Journal of Biomedical Science·Chih-Wei TungShang-Hsun Yang

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Methods Mentioned

BETA
light scattering
mechanical dissociation
transfection
fluorescence microscopy

Software Mentioned

ImageJ
GraphPad Prism

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