MicroRNA-125a-5p modulates human cervical carcinoma proliferation and migration by targeting ABL2

Drug Design, Development and Therapy
Xian QinMin Xue

Abstract

In this study, we intended to understand the regulatory mechanisms of microRNA-125a-5p (miR-125a-5p) in human cervical carcinoma. The gene expressions of miR-125a-5p in seven cervical carcinoma cell lines and 12 human cervical carcinoma samples were evaluated by quantitative real-time reverse transcription polymerase chain reaction. Ca-Ski and HeLa cells were transduced with lentivirus carrying miR-125a-5p mimics, and the effects of lentivirus-induced miR-125a-5p upregulation on cervical carcinoma proliferation and migration were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and transwell assays, respectively. In additional, HeLa cells were inoculated into null mice to evaluate the effect of miR-125a-5p upregulation on in vivo cervical carcinoma growth. The direct regulation of miR-125a-5p on its target gene, ABL proto-oncogene 2 (ABL2), in cervical carcinoma was evaluated by quantitative real-time reverse transcription polymerase chain reaction, Western blotting and luciferase reporter assays, respectively. ABL2 was then downregulated by small interfering RNA to examine its effect on cervical carcinoma proliferation and migration. miR-125a-5p was downregulated in both cervical carcinoma cell lines an...Continue Reading

Citations

Jul 26, 2017·Genes & Cancer·Muralidharan JayaramanDanny N Dhanasekaran
Mar 7, 2018·Oncotarget·Sampa Ghoshal-GuptaMumtaz V Rojiani
Nov 19, 2020·Journal of Clinical Medicine·Justyna Pisarska, Katarzyna Baldy-Chudzik
May 29, 2021·Environmental Science and Pollution Research International·Marwa M MahmoudNadia M Hamdy

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Methods Mentioned

BETA
PCR
transfection

Software Mentioned

TargetScan
GraphPad Prism6

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