MicroRNA-126 is a regulator of platelet-supported thrombin generation.

Platelets
Veronika ZapilkoPierre Fontana

Abstract

Circulating microRNA (miRNA) expression profiles correlate with platelet reactivity. MiR-126 is a promising candidates in this regard. We generated a transgenic zebrafish line with thrombocyte-specific overexpression of miR-126. Laser injury of the posterior cardinal vein of 5 day-old larvae was performed with or without antithrombotic pre-treatment. Platelet-like structures (PLS) derived from human megakaryocytes transfected with miR-126 were also evaluated for procoagulant activity. Finally, we studied the correlation between miR-126 level and thrombin generation markers in a cohort of stable cardiovascular patients. Control zebrafish developed small thrombocyte-rich thrombi at the site of vessel injury, without vessel occlusion. The miR-126 transgenic line developed an occluding thrombus in 75% (95% CI: 51-91%) of larvae. Pre-treatment with the direct thrombin inhibitor argatroban, but not aspirin, prevented vessel occlusion in the transgenic line (0% occlusion, 95%CI: 0-18%). Upon activation, human PLS showed an increased procoagulant profile after miR-126 transfection compared to control. Finally, the plasma levels of miR-126, but not a control platelet-derived miRNA, correlated with markers of in vivo thrombin generation ...Continue Reading

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Citations

Oct 31, 2020·Thrombosis and Haemostasis·Alix GarciaPierre Fontana
May 4, 2021·Frontiers in Physiology·Pamela CzajkaMarek Postula
Jun 2, 2021·Clinical Research in Cardiology : Official Journal of the German Cardiac Society·Andreas ZietzerFelix Jansen
Jul 1, 2021·Hämostaseologie·A GarciaP Fontana

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