MicroRNA-126 Overexpression Inhibits Proliferation and Invasion in Osteosarcoma Cells

Technology in Cancer Research & Treatment
Shuai-Hua WangWen He

Abstract

This study investigated the biological effects of microRNA-126 overexpression in human MG63 osteosarcoma cells. A recombinant plasmid expressing microRNA-126, pcDNA6.2-microRNA-126, was constructed and transfected into MG63 cells. Using real-time fluorogenic quantitative polymerase chain reaction, the microRNA-126 expression was measured in microRNA-126-MG63 group, Ctrl-MG63 group, and blank group. Cell proliferation, cell cycle distribution, cell migration, and invasion were analyzed using methyl thiazolyl tetrazolium assay, flow cytometer, wound-healing assay, and transwell assay, respectively. As expected, microRNA-126 expression was higher in microRNA-126-MG63 group than in Ctrl-MG63 group and blank group (both P < .05). After 48/72 hours of transfection, cell proliferation in microRNA-126-MG63 group was significantly reduced compared to blank group (both P < .05). Compared to blank group, cell population in G0/G1 stage was significantly higher in microRNA-126-MG63 group, accompanied by lower cell numbers in the S and G2/M phases and decreased proliferation index (all P < .05). Wound-healing assay showed a wider scratch width in microRNA-126-MG63 group and reduced cell migration than blank group (both P < .05). Cells overex...Continue Reading

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Citations

Apr 6, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·G M VieraM S Brassesco
Mar 20, 2018·Cellular and Molecular Life Sciences : CMLS·Victor O OriaOliver Schilling
Jun 8, 2021·Molecular Therapy Oncolytics·Zahra Sadat RazaviHamed Mirzaei

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Methods Mentioned

BETA
Transfection
flow cytometry
antisense oligonucleotides
PCR
transfections
enzyme-linked immunosorbent assay
protein assay
electrophoresis
Assay

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )
Basic Local Alignment Search Tool
TargetScan
Gene Tools
SPSS
Chromas

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