MicroRNA-127-3p controls murine hematopoietic stem cell maintenance by limiting differentiation

Haematologica
Laura CrisafulliFrancesca Ficara

Abstract

The balance between self-renewal and differentiation is crucial to ensure the homeostasis of the hematopoietic system, and is a hallmark of hematopoietic stem cells. However, the underlying molecular pathways, including the role of micro-RNA, are not completely understood. To assess the contribution of micro-RNA, we performed micro-RNA profiling of hematopoietic stem cells and their immediate downstream progeny multi-potent progenitors from wild-type control and Pbx1-conditional knockout mice, whose stem cells display a profound self-renewal defect. Unsupervised hierarchical cluster analysis separated stem cells from multi-potent progenitors, suggesting that micro-RNA might regulate the first transition step in the adult hematopoietic development. Notably, Pbx1-deficient and wild-type cells clustered separately, linking micro-RNAs to self-renewal impairment. Differential expression analysis of micro-RNA in the physiological stem cell-to-multi-potent progenitor transition and in Pbx1-deficient stem cells compared to control stem cells revealed miR-127-3p as the most differentially expressed. Furthermore, miR-127-3p was strongly stem cell-specific, being quickly down-regulated upon differentiation and not re-expressed further dow...Continue Reading

Citations

Feb 23, 2020·International Journal of Molecular Sciences·Viviana ScalavinoGrazia Serino
Jun 9, 2020·Mechanisms of Ageing and Development·Daniëlle Gaby Luinenburg, Gerald de Haan
Sep 18, 2021·Wiley Interdisciplinary Reviews. RNA·Laura Crisafulli, Francesca Ficara

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Methods Mentioned

BETA
Assay
Fluorescence
PCR
FACS

Software Mentioned

Statistical Analysis of Microarray 8 ( SAM )
GraphPad Prism
GraphPad

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