MicroRNA-128-3p Enhances the Chemosensitivity of Temozolomide in Glioblastoma by Targeting c-Met and EMT.

Scientific Reports
Chengbin ZhaoBo Yang

Abstract

Temozolomide is a first line anti-tumor drug used for the treatment of patients with Glioblastoma multiforme (GBM). However, the drug resistance to temozolomide limits its clinical application. Therefore, novel strategies to overcome chemoresistance are desperately needed for improved treatment of human GBM. Recent studies have demonstrated that miRNAs are closely related to resistance to cancer chemotherapy. This study aimed to further validate the biological role of miR-128-3p and to investigate whether miR-128-3p can enhance the chemosensitivity of glioblastoma to temozolomide (TMZ) and the underlying mechanisms. The effects of miR-128-3p and TMZ on the proliferation of glioblastoma cells were investigated by cell counting kit-8 (cck8). Transwell and intracerebral invasion assays were applied to determine the effects of the combination of miR-128-3p and TMZ on the invasion and migration of glioblastoma in vitro and in vivo. Flow cytometry was used to detect apoptosis in each group, and immunofluorescence was used to determine the expression levels of EMT-related proteins. RT-PCR and Western-blot were applied to detect EMT-transformed proteins (c-Met, PDGFRα, Notch1, and Slug) and EMT phenotype-associated proteins (Vim, CD44,...Continue Reading

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Citations

Aug 18, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Zhi Xiong ChongWan Yong Ho
Aug 23, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Manendra Singh TomarAshutosh Shrivastava

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Methods Mentioned

BETA
transfection
protein
electrophoresis
flow cytometry
Assay

Software Mentioned

Digimizer
TargetScan
SPSS
Image J

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