MicroRNA-128 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells by repressing Sp1

Molecular and Cellular Biochemistry
Yang DaiHong Guo

Abstract

MicroRNAs (miRNAs) play essential roles in muscle cell proliferation and differentiation. The muscle-specific miRNAs miR-1 and miR-206 have been shown to regulate muscle development and promote myogenic differentiation; however, it is likely that a number of other miRNAs play important roles in regulating myogenesis as well. microRNA-128 (miR-128) has been reported to be highly expressed in brain and skeletal muscle, and we found that miR-128 is also up-regulated during bovine skeletal muscle satellite cell differentiation using microarray analysis and qRT-PCR. However, little is known about the functions of miR-128 in bovine skeletal muscle satellite cell development. In this study, we investigated the biological functions of miR-128 in bovine skeletal muscle cell development. Using a dual-luciferase reporter assay, we confirmed that miR-128 regulates the Sp1 gene. Over-expression of miR-128 reduced Sp1 protein levels and inhibited muscle satellite cell proliferation and differentiation. Inhibition of miR-128 increased Sp1 protein levels and promoted muscle satellite cell differentiation but also suppressed proliferation. Changes in miR-128 and Sp1 expression levels also affected the protein levels of MyoD and CDKN1A. Sp1, an ...Continue Reading

References

Apr 1, 1992·Trends in Genetics : TIG·M Buckingham
Dec 1, 1990·Current Opinion in Cell Biology·C P Emerson
Sep 1, 1990·Genes & Development·E N Olson
May 22, 2001·Cellular and Molecular Life Sciences : CMLS·M Kitzmann, A Fernandez
Apr 19, 2003·The Journal of Biological Chemistry·Chang Sheng GuoMathew J Thayer
Sep 17, 2004·Nature·Gunter Meister, Thomas Tuschl
Apr 23, 2005·The European Journal of Neuroscience·Lena SmirnovaF Gregory Wulczyn
Feb 21, 2006·Nature Cell Biology·Irina NaguibnevaAnnick Harel-Bellan
May 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Prakash K RaoHarvey F Lodish
Aug 23, 2006·The Journal of Cell Biology·Hak Kyun KimAnindya Dutta
Feb 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Robert W GeorgantasCurt I Civin
Feb 19, 2008·The Journal of Biological Chemistry·Chung Fai Wong, Ross L Tellam
Mar 8, 2008·Trends in Genetics : TIG·Eva van RooijEric N Olson
May 7, 2008·Trends in Molecular Medicine·Thomas E Callis, Da-Zhi Wang
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Colin G CristMargaret Buckingham
Jan 5, 2011·The Journal of Cell Biology·Yejing GeJie Chen
May 2, 2012·The Journal of Biological Chemistry·Jing ZhangKui Li
Apr 23, 2013·Journal of Cell Science·Norio MotohashiLouis M Kunkel

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Citations

Sep 30, 2016·Frontiers in Physiology·Stavroula TsitkanouAaron P Russell
Nov 2, 2016·In Vitro Cellular & Developmental Biology. Animal·Wei Ran ZhangHong Guo
Feb 23, 2019·Journal of Cellular Physiology·Chengchuang SongHong Chen
Mar 17, 2020·Journal of Animal Science·Madison L GonzalezSally E Johnson
Aug 17, 2019·International Journal of Molecular Sciences·Yinghui LingXiaorong Zhang
Nov 27, 2020·Journal of Cellular Physiology·Qi ShangHui Ren
Dec 10, 2020·Critical Reviews in Food Science and Nutrition·Anshuman SinghRajneesh Jha
Jul 20, 2019·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Li LiHongping Zhang
Dec 30, 2020·Biology·Sílvia C RodriguesFilipe V Duarte

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