Sep 10, 2015

MicroRNA-128 suppresses cell growth and metastasis in colorectal carcinoma by targeting IRS1

Oncology Reports
Lan WuGuoyu Fan

Abstract

Evidence has shown that microRNAs play important roles in tumor development, progression, and metastasis. miR-128 has been reported to be deregulated in different tumor types, whereas the function of miR-128 in colorectal carcinoma (CRC) largely remains to be elucidated. The aim of the present study was to investigate the clinical significance, biological effects and underlying mechanisms of miR-128 in CRC using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. It was found that the expression of miR-128 was downregulated in CRC tissues and cell lines as determined by RT-qPCR. Furthermore, the expression of miR-128 in tumor tissues was significantly negatively correlated with TNM stage and lymph node metastasis in CRC patients. Functional assay revealed that the overexpression of miR-128 inhibited CRC cell proliferation, colony formation, migration and invasion and promoted apoptosis in vitro, and suppressed CRC xenograft tumor growth in vivo. In addition, insulin receptor substrate 1 (IRS1), a key mediator in oncogenic insulin-like growth factor (IGF) signaling, was confirmed as a direct target of miR-128 by a luciferase reporter assay. Western blot analysis indicated that the overexp...Continue Reading

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Study
In Vivo
Gene Expression Regulation, Neoplastic
IRS1 protein, human
Tumor Suppressor Genes
TNM Staging System
AKT1
Cell Motility
Tumor Tissue Sample

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