MicroRNA-129-5p inhibits the development of autoimmune encephalomyelitis-related epilepsy by targeting HMGB1 through the TLR4/NF-kB signaling pathway

Brain Research Bulletin
Ai-Hua LiuYu-Ping Wang

Abstract

The study aimed to explore the effects of microRNA-129-5p (miR-129-5p) on the development of autoimmune encephalomyelitis (AE)-related epilepsy by targeting HMGB1 through the TLR4/NF-kB signaling pathway in a rat model. AE-related epilepsy models were established. Sprague-Dawley (SD) rats were randomly divided into control, model, miR-129-5p mimics, miR-129-5p inhibitor, HMGB1 shRNA, TLR4/NF-kB (TLR4/NF-kB signaling pathway was inhibited) and miR-129-5p mimics+HMGB1 shRNA groups respectively. Latency to a first epilepsy seizure attack was recorded. Neuronal injuries in the hippocampus regions were detected using HE, Nissl and FJB staining methods 24h following model establishment. Microglial cells were detected by OX-42 immunohistochemistry. Expressions of miR-129-5p, HMGB1 and TLR4/NF-kB signaling pathway-related proteins were detected by qRT-PCR. Protein expressions of HMGB1 and TLR4/NF-kB signaling pathway-related proteins were detected by Western blotting. Dual luciferase reporter gene assay showed that miR-129-5p was negatively targeting HMGB1. Neurons of hippocampal tissues in rats were heavily injured by an injection of lithium chloride. Compared with the model and control groups, neuronal injury of the hippocampus and A...Continue Reading

Citations

Dec 25, 2019·Genes & Genomics·Chenglong LiuFei Zhang
Jun 2, 2020·Frontiers in Cellular Neuroscience·Ximena Paez-ColasanteEva L Feldman
Apr 9, 2020·International Journal of Molecular Sciences·Yam Nath PaudelMohd Farooq Shaikh
Jul 18, 2019·Frontiers in Neurology·Dominik KobylarekWojciech Kozubski
May 13, 2020·Arthritis Research & Therapy·Ji-Na ZhengQiang Wang
Dec 15, 2020·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Weiye XuFafa Tian
Jan 14, 2021·Immunopharmacology and Immunotoxicology·Maryam NematiMohammad Nouri
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Jun 5, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMajid Mokhtari
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