MicroRNA-134 prevents the progression of esophageal squamous cell carcinoma via the PLXNA1-mediated MAPK signalling pathway

EBioMedicine
Wei-Wei WangXiang-Nan Li

Abstract

MicroRNAs (miRNAs) are involved in oncogenesis of esophageal squamous cell carcinoma (ESCC). miR-134 is reported to have a tumour-suppressive role but its role in ESCC is not known. The present study was designed to examine whether miR-134 inhibits ESCC development and further explored relevant underlying mechanisms. Differentially expressed genes related to ESCC were identified from microarray gene expression profiles. Immunohistochemical staining and RT-qRCR assays identified elevated PLXNA1 expression levels and low miR-134. The relationship between miR-134 and PLXNA1 was predicted and further verified by a dual-luciferase reporter assay. The expression levels of miR-134 and PLXNA1 in ESCC cells were modified by miR-134 mimic/inhibitor and siRNA against PLXNA1, respectively. Thereafter, the expression of MAPK signalling pathway-related proteins, as well as the viability, migration, invasion, cell cycle and cell apoptosis of ESCC cells was investigated. The results showed that miR-134 could block the MAPK signalling pathway by downregulating PLXNA1. When miR-134 was overexpressed or PLXNA1 was silenced, cell apoptosis was enhanced, the cell cycle was retarded, and the cell proliferation, migration and invasion were suppressed...Continue Reading

Citations

Aug 24, 2021·Journal of Receptor and Signal Transduction Research·Pengbo Li, Gang Huang

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Datasets Mentioned

BETA
GSE45168
GPL13497
GSE45670
GPL570
GSE20347
GSE29001
GSE17351

Methods Mentioned

BETA
biopsies
surgical resection
transfection
PCR
electrophoresis
X ray
flow cytometry
xenograft

Software Mentioned

miRDB
TargetScan
R
miRWalk
MicroRNA
Pro Plus Analysis
limma
Image
Venn
pheatmap

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