MicroRNA-135b regulates metastasis suppressor 1 expression and promotes migration and invasion in colorectal cancer

Molecular and Cellular Biochemistry
Weiyun WuYu Zhou

Abstract

MicroRNAs (miRNAs, miRs) play important roles in pathogenesis and development of human diseases, including malignancy. Some may affect tumor progression through targeting tumor suppressor genes. MiR-135b has been shown to be upregulated in CRC. In this study, we evaluated the role of miR-135b in colorectal cancer (CRC) and its regulatory role for metastasis suppressor-1 (MTSS1) and its mechanisms. The levels of miR-135b and MTSS1 gene expression in 35 CRC and corresponding cancer-adjacent tissues, 27 colorectal adenoma, and 16 normal tissue samples were quantified using qRT-PCR and western blot analysis. The effect of miR-135b on MTSS1 expression was assessed by miR-135b mimics or inhibitor transfection to deregulate miR-135b expression. The direct interaction between them was verified by 3'-UTR dual-luciferase reporter assay. Furthermore, the roles of miR-135b in regulating CRC cells migration and invasion properties were analyzed with miR-135b mimics or inhibitor-transfected cells and silenced expression of MTSS1 in miR-135b inhibitor transfected cells. CRC tissues showed significantly upregulated miR-135b expression and reduced MTSS1 expression. High miR-135b levels were significantly associated with lymph node and distant m...Continue Reading

References

Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Oct 10, 2006·Carcinogenesis·Hirotaka Osada, Takashi Takahashi
Mar 31, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Christian Parr, Wen G Jiang
Jul 28, 2009·Annual Review of Medicine·Ramiro GarzonCarlo M Croce
Mar 23, 2010·Lancet·David CunninghamNaureen Starling
Oct 5, 2010·Nature Reviews. Drug Discovery·Ramiro GarzonCarlo M Croce
Nov 18, 2010·Carcinogenesis·William K K WuJoseph J Y Sung
Jun 1, 2012·The Cancer Journal·Aaron J SchetterCurtis C Harris
Jun 22, 2012·Journal of Translational Medicine·Muhammad Imran AslamJames Howard Pringle
Jan 1, 2011·Experimental and Therapeutic Medicine·Noha MustafaWen G Jiang
Sep 29, 2012·Acta Biochimica Et Biophysica Sinica·Weiwei ZhouShaojun Liu
Apr 30, 2013·The American Journal of Pathology·Maddalena ArigoniElena Quaglino
May 18, 2013·Journal of Translational Medicine·Peng QiXiang Du

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Citations

Feb 2, 2017·Oncotarget·Ann E ZeleniakReginald Hill
Nov 25, 2014·Molecular and Cellular Biochemistry·Hong PeiWeichun Guo
Jul 17, 2015·International Journal of Cancer. Journal International Du Cancer·Irfete S FetahuEnikő Kállay
Jul 25, 2018·World Journal of Gastroenterology : WJG·Kenneth Kw ToWilliam Cs Cho
Mar 12, 2016·Journal of Experimental & Clinical Cancer Research : CR·Yudan Chi, Dongming Zhou
Dec 17, 2015·Expert Review of Molecular Diagnostics·Alessandro CanellaFlavia Pichiorri
Jan 2, 2019·World Journal of Gastroenterology : WJG·Marcela Alcântara ProençaAna Elizabete Silva
Oct 28, 2019·Journal of Cellular Physiology·Zohreh JahanafroozBehzad Baradaran
Dec 2, 2015·Leukemia·M SchemionekS Koschmieder
Jul 23, 2015·Pathology Oncology Research : POR·Li ZhouYu-Pei Zhao
Dec 12, 2018·Proceedings of the National Academy of Sciences of the United States of America·Alexander S BrownAnthony E Oro
May 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wei SongJu Liu

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