MicroRNA-136-3p inhibits glioma tumorigenesis in vitro and in vivo by targeting KLF7.

World Journal of Surgical Oncology
Yanwu Xu

Abstract

Malignant brain tumors have been a serious threat to human health worldwide. This study aims to investigate the role of miR-136-3p in glioma development. Hematoxylin-eosin staining (H&E) staining was used to determine the pathologic alterations of glioma tissues. Quantitative real-time PCR (qRT-PCR) analysis and GEO2R analysis was performed to examine the expression of miRNAs and genes. Western blot was applied to detect the protein expression. Cell counting kit-8 (CCK-8) and colony formation were used to analyze the glioma cell growth. Trans-well assay was used to determine the cell migration. Annexin V-FITC/PI staining was conducted to determine the cell apoptosis of transfected glioma cells. The dual-luciferase reporter assay was carried out to confirm the binding sites of miR-136-3p on 3' untranslated regions (3' UTR) of Kruppel-like factor 7 (KLF7). Tumor-bearing experiment in nude mice was performed to comprehensively investigate the role of miR-136-3p/KLF7 axis in gliomas. Firstly, the results showed that miR-136-3p was decreased in glioma tissues compared with adjacent tissues. Overexpression of miR-136-3p significantly inhibited cell growth of LN-229 and U251 by decreasing expression of Cyclin A1 and PCNA (proliferatin...Continue Reading

References

Feb 23, 2010·Genomics, Proteomics & Bioinformatics·Yimei CaiJun Yu
Oct 3, 2012·Trends in Cell Biology·Masashi Abe, Nancy M Bonini
Sep 24, 2015·Oncology Reports·Bijing Mao, Ge Wang
Oct 1, 2015·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Otto VisserUNKNOWN EUROCARE-5 Working Group:
Aug 30, 2016·MicroRNA·Wen-Juan Ni, Xiao-Min Leng
Oct 26, 2016·International Journal of Oncology·Kirsten HattermannJanka Held-Feindt
Oct 5, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zhonghua JiangXin Lin
Aug 1, 2018·Lancet·Sarah LapointeNicholas A Butowski
Apr 26, 2019·Biochemical and Biophysical Research Communications·Feng GuanZhi-Qiang Hu
Jun 1, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yun-Xia AnXiao-Ju Zhang
Feb 18, 2020·Cancer Cell International·Juan YaoKun Zhao

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Citations

Feb 10, 2021·International Journal of Molecular Sciences·Shalini Das GuptaAsla Pitkänen

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Datasets Mentioned

BETA
GSE103228

Methods Mentioned

BETA
electrophoresis
transfection
flow cytometry
trans-well
transfecting

Software Mentioned

GraphPad Prism
GEO2R
Graphpad
TargetScan

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