MicroRNA-138 negatively regulates the hypoxia-inducible factor 1α to suppress melanoma growth and metastasis

Biology Open
haijiang qiuMinjun Chen

Abstract

Melanoma with rapid progression towards metastasis has become the deadliest form of skin cancer. However, the mechanism of melanoma growth and metastasis is still unclear. Here, we found that miRNA-138 was lowly expressed and hypoxia-inducible factor 1α (HIF1α) was highly expressed in patients' melanoma tissue compared with the paracancerous tissues, and they had a significant negative correlation (r=-0.877, P<0.001). Patients with miRNA-138low/HIF1αhigh signatures were predominant in late stage III/IV of melanoma. Further, bioinformatic analysis demonstrated that miRNA-138 directly targeted HIF1α. We found that the introduction of pre-miRNA-138 sequences to A375 cells reduced HIF1α mRNA expression and suppressed cell proliferation, migration and invasion. Overexpression of miRNA-138 or inhibition of HIF1α significantly suppressed the growth and metastasis of melanoma in vivo Our study demonstrates the role and clinical relevance of miRNA-138 and HIF1α in melanoma cell growth and metastasis, providing a novel therapeutic target for suppression of melanoma growth and metastasis.

References

Feb 26, 2008·Nature Cell Biology·Muh-Hwa YangKou-Juey Wu
Mar 5, 2008·Cancer Research·Lynne T BemisWilliam A Robinson
Feb 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Miguel F SeguraEva Hernando
Dec 19, 2009·Neoplasia : an International Journal for Oncology Research·Chi-Hung HuangMuh-Hwa Yang
Sep 10, 2010·Melanoma Research·Martin GludKrzysztof T Drzewiecki
Mar 11, 2011·Cancer Biology & Therapy·Jian JiangXin-hua Liang
Apr 2, 2011·The Journal of Biological Chemistry·Altaf A DarMohammed Kashani-Sabet
Jun 23, 2011·Molecular Cancer Research : MCR·Yemin WangToshiyasu Taniguchi
Aug 13, 2011·The Journal of Veterinary Medical Science·Shunsuke NoguchiKohji Maruo
Sep 7, 2011·The American Journal of Pathology·Jiamin ChenVictor A Tron
Jan 25, 2013·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Huijun ZhangKe Fei
Feb 8, 2013·International Journal of Cancer. Journal International Du Cancer·Yu-Ming YehLu-Hai Wang
Mar 12, 2013·The Journal of Investigative Dermatology·Daniel S WidmerMitchell P Levesque
Apr 16, 2015·Cellular Oncology (Dordrecht)·Chao YuChengyi Sun
Jun 20, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Doug HannifordEva Hernando
Oct 27, 2015·Cancer & Metabolism·Laura SchöckelMélanie Héroult
Jan 6, 2016·Melanoma Research·Gerald SaldanhaJames H Pringle
Jan 15, 2016·The Journal of Investigative Dermatology·Kaushala JayawardanaJean Yang
Jan 23, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun ZhangLianhong Li
Apr 21, 2016·Journal of Experimental & Clinical Cancer Research : CR·Baoen JiangHongyan Tian
Jun 29, 2016·Molecular Diagnosis & Therapy·Zhenjun DengLi He
Feb 9, 2017·Pigment Cell & Melanoma Research·Stacie K LoftusWilliam J Pavan
Jan 18, 2018·Immunology·Jeffrey L EbersoleOctavio A Gonzalez
Sep 6, 2018·Biochemical and Biophysical Research Communications·Heling JiaoWei Zhang

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Citations

Feb 6, 2020·International Journal of Molecular Sciences·Francesca Varrone, Emilia Caputo
Aug 11, 2020·Journal of Receptor and Signal Transduction Research·Weina ZhangJizhen Ren
Oct 2, 2020·Technology in Cancer Research & Treatment·Saijun LiuLiehua Deng
May 1, 2021·International Journal of Molecular Sciences·Masashi IshikawaDaqing Ma
May 11, 2021·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
Jul 6, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohammad MalekanFateme Sheida

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Methods Mentioned

BETA
flow cytometry
transfection
PCR
surgical resection
Infrared Imaging

Software Mentioned

SPSS12

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