MicroRNA-139-5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β-catenin signaling pathway

Experimental and Therapeutic Medicine
Yufei Xie, Gang Shen

Abstract

The aim of the present study was to identify a microRNA (miRNA or miR)-based biomarker and therapeutic target for skeletal myogenic differentiation of human adult dental pulp stem cells (ADSCs). miRNA expression was measured using reverse transcription-quantitative polymerase chain reaction; cell viability assay and lactate dehydrogenase (LDH) activity levels were measured using MTT and LDH activity kits, respectively. Apoptosis assay and caspase-3/9 activity levels were measured using flow cytometry and caspase-3/9 activity kits, respectively. Western blot analysis and immunofluorescence microscopy measured the protein expression of myocyte-specific enhancer factor 2C, myogenic differentiation 1, myosin heavy chain, Wnt and β-catenin. Overexpression of miR-139-5p promoted cell growth and induced skeletal myogenic differentiation of ADSCs. Downregulation of miR-139-5p inhibited cell growth and reduced skeletal myogenic differentiation of ADSCs. Overexpression of miR-139-5p induced Wnt/β-catenin signaling pathway and Wnt/β-catenin signaling pathway was suppressed by anti-miR-139-5p in ADSCs. Wnt inhibitor reduced the effect of miR-139-5p on skeletal myogenic differentiation of ADSCs. In conclusion, miR-139-5p elevates skeletal m...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 17, 2014·World Journal of Diabetes·Awadhesh K AryaKamlakar Tripathi
Dec 23, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Lin MiXueli Zheng
Aug 20, 2015·Journal of Foot and Ankle Research·Agnetha FolestadJean Cassuto
Sep 10, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhao-Hui WangDong-Lin Zheng
Nov 15, 2015·The Journal of Pharmacology and Experimental Therapeutics·Eryun ZhangZhengtao Wang
Mar 6, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Liping ZhuOscar A Carretero
Mar 8, 2016·Nature Communications·Defang LiGe Zhang
May 10, 2017·Diabetes Research and Clinical Practice·Xueyao YinHong Li
Jul 20, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hongbo LuoChuanrui Xu

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Citations

Dec 5, 2019·Frontiers in Cell and Developmental Biology·Rui-Juan ChengTony N Marion
Nov 6, 2020·Stem Cells International·Dan ZhouWei Du

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Methods Mentioned

BETA
PCR
electrophoresis
transfection
Assay

Software Mentioned

Flowjo
Image Lab

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