MicroRNA-141 inhibits proliferation of gastric cardia adenocarcinoma by targeting MACC1

Archives of Medical Science : AMS
Shubin LiBin Li

Abstract

Abnormal expression of microRNAs (miRNAs) contributes to cancer development through regulating proliferation, apoptosis and drug resistance in cancer cells. The present study was designed to explore the effect and mechanism of miR-141 on gastric cardia adenocarcinoma (GCA). Forty-one paired GCA tissues and adjacent normal tissues were obtained from GCA patients never treated by chemotherapy or radiotherapy. QRT-PCR was used to detect the expression level of miR-141 in GCA. Effects of miR-141 on cell proliferation and apoptosis were detected in vitro. Western blot analysis was used to determine the downstream targets of miR-141. In the present study, our data showed that miR-141 was significantly decreased and correlated with advanced TNM stage and lymph node metastases in GCA. In addition, we found that miR-141 could inhibit cell proliferation and induce cell apoptosis in AGS cells. Moreover, MACC1 was predicted as a possible target of miR-141. The luciferase reporter assay proved that miR-141 could suppress MACC1 directly by binding to its 3'-UTR. Further studies showed that miR-141 influenced the MEK/ERK and p38 MAPK signaling pathways. Our findings demonstrated that miR-141 expression was associated with GCA progression. MAC...Continue Reading

Citations

Jul 19, 2019·Experimental and Therapeutic Medicine·Chunhua ZhangXiaoqian Hou
Jan 5, 2019·Cancer Metastasis Reviews·Harikrishnan RadhakrishnanUlrike Stein
Jan 10, 2021·Life Sciences·Sepideh MirzaeiMichael R Hamblin
May 11, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yonghong WangXiaowei Li

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Methods Mentioned

BETA
PCR
transfection
Assay
protein assay
flow cytometry

Software Mentioned

GraphPad Prism
SPSS
miRwalk
miRanda
Quantity One
ModFit
TargetScan

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