MicroRNA-146a attenuates the development of morphine analgesic tolerance in a rat model

Neurological Research
Ying WangYan Wang

Abstract

Background: Morphine plays an irreplaceable role in relieving severe pain clinically, while long-term medication inevitably leads to drug resistance. MicroRNA (miR) 146a has been reported to be a negative regulator in the process of morphine-tolerance formation. This study aimed to investigate how miR-146a affects the development of morphine analgesic tolerance.Methods: The morphine-tolerance rat model was established by means of one-week continuous morphine administration. Paw withdrawal latency test was performed every day, and spinal cord samples were dissected on the seventh day for Q-PCR and Western blotting to detect the expression level of miR-146a, and IRAK1/TRAF6 participated in TLR4 signaling pathway.Results: The expression of miR-146 was significantly decreased in morphine-tolerant model. Also, overexpression of miR-146a reduced the resistance caused by morphine, followed by the down-regulation of IRAK1/TRAF6 in TLR4 pathway. The inhibition of miR-146a remarkably decreased paw withdrawal latency as well as increased the expression levels of TLR4 signaling pathway-related molecules, IRAK1 and TRAF6.Conclusion: This study suggests that miR-146a attenuates morphine tolerance by inhibiting the expression of IRAK1/TRAF6 i...Continue Reading

References

Jun 15, 2007·Journal of Neuroimmunology·Christine BörnerJürgen Kraus
Feb 12, 2013·The Journal of Pain : Official Journal of the American Pain Society·Lori N Eidson, Anne Z Murphy
Apr 30, 2013·Cell·Luke A YatesRobert J C Gilbert
Aug 14, 2018·International Immunopharmacology·Xiaozhi BaiDahai Hu
Feb 16, 2019·PLoS Genetics·Marah C RuntschRyan M O'Connell
Jun 11, 2019·International Journal of Medical Microbiology : IJMM·Nanthapon RuangkiattikulRalph Goethe

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