MicroRNA-146a induction during influenza H3N2 virus infection targets and regulates TRAF6 levels in human nasal epithelial cells (hNECs)

Experimental Cell Research
Yuqin DengDe-Yun Wang

Abstract

We have previously shown that human nasal epithelial cells (hNECs) are highly permissive cells for respiratory viruses including influenza A virus (IAV) and respiratory syncytial virus. Recent studies have indicated that microRNAs (miRNAs) are involved in virus-host relationship, and this led us to investigate its essential roles in the in vitro hNECs model derived from multiple donors. By comparing the differential expression of miRNAs upon IAV infection among animal and cell line studies, candidates were selected with focus on the initial immune response. After infection of influenza H3N2 virus, hNECs showed constant increase virus titer at 24-72h post-infection (hpi); accompanied with a significantly elevated level of miR-146a-5p at 72 hpi. The exponential elevation of progeny virus titer correlated with a key influenza sensing Toll-like receptor (TLR)7 pathway. TLR7 downstream gene transcripts, myeloid differentiation primary response gene 88 (MyD88), interferon regulator factor 7 (IRF7), and interferon-β (IFN-β) were significantly upregulated at 48 and 72 hpi, while interleukin-1 receptor-associated kinase 1 (IRAK1) and TNF receptor associated factor-6 (TRAF6) were unchanged. Interestingly, when miR-146a was overexpressed ...Continue Reading

References

Apr 9, 2017·Current Allergy and Asthma Reports·Kai Sen TanDe-Yun Wang
Apr 25, 2018·The FEBS Journal·Charlotte NejadMichael P Gantier
Jun 12, 2018·Epigenomics·Mohsen KeshavarzHamed Mirzaei
Jan 1, 2020·IUBMB Life·Javid Sadri NahandHamed Mirzaei
Jun 3, 2020·International Journal of Molecular Sciences·Nilay MitashAgnieszka Swiatecka-Urban
Jul 20, 2018·Frontiers in Immunology·Thi Hiep NguyenMing Yang
Nov 1, 2020·Allergy·J WeidnerM Rådinger

Citations

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 15, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Emmie de Wit, Ron A M Fouchier
Jul 15, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jin HouXuetao Cao
Jan 15, 2010·Journal of Virology·Yu LiMichael G Katze
Sep 18, 2010·Cell and Tissue Research·Catherine J SandersPaul G Thomas
Oct 13, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pin WangXuetao Cao
Nov 18, 2011·Molecular Biology International·Nicole Rusca, Silvia Monticelli
Mar 1, 2012·Science·Ruslan MedzhitovMiguel P Soares
Jul 24, 2012·The Journal of Biological Chemistry·William A BuggeleCurt M Horvath
Jun 6, 2013·The Journal of Allergy and Clinical Immunology·Thomas X Lu, Marc E Rothenberg
Nov 19, 2013·The Journal of General Virology·Carrie M RosenbergerAlan Aderem
Dec 4, 2013·Chest·Christopher L Grainge, Donna E Davies
May 3, 2014·Expert Review of Clinical Immunology·Anna GłobińskaMarek L Kowalski
Jan 13, 2015·Organic & Biomolecular Chemistry·Tian TianXiang Zhou
Mar 10, 2015·The American Journal of Pathology·John C Kash, Jeffery K Taubenberger
May 10, 2015·Brain, Behavior, and Immunity·Ying LuYong-Jing Gao
Oct 16, 2015·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hiroshi Kido
Nov 1, 2015·Experimental Biology and Medicine·Jarika MakkochSunchai Payungporn
Dec 3, 2015·Mucosal Immunology·M WeitnauerA H Dalpke
Feb 13, 2016·Frontiers in Physiology·Steven MaltbyPaul S Foster
Sep 10, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Niannian Fan, Jiantao Wang

Related Concepts

Computer Software
IRAK1 gene
Real-Time Polymerase Chain Reaction
Respiratory Syncytial Virus Infections
Study
Immune Response
Immunofluorescence Assay
Biochemical Pathway
TLR7 protein, human
Nasal Epithelium

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.