MicroRNA-146a suppresses rheumatoid arthritis fibroblast-like synoviocytes proliferation and inflammatory responses by inhibiting the TLR4/NF-kB signaling

Oncotarget
Wei LiuYang Ji

Abstract

This study investigated whether microRNA-146a (miR-146a) mediating TLR4/NF-κB pathway affected proliferation and inflammatory responses of rheumatoid arthritis fibroblast-like synoviocytes from 12 RA patients (RA-FLSs). FLSs in the logarithmic growth phase were assigned into the control, miR-146a mimic miR-146a inhibitor, Tak-242 (treated with TLR4/NF-κB pathway inhibitor) and mimic + lipopolysaccharide (LPS) groups. Cell proliferation and apoptosis were detected using CCK-8 assay and flow cytometry. The expression of miR-146a, TLR4/NF-κB pathway-related proteins and cytokines were determined by RT-qPCR, western blotting and ELISA, and the release of NO by Greiss reaction. RA rat models were constructed and the primary cells were classified into the control, negative control (NC), miR-146a mimic, miR-146a inhibitor, Tak-242, mimic + LPS, and TLR4 groups. Immunohistochemistry was used to detect the expression of proliferating cell nuclear antigen (PCNA) and intercellular adhesion molecular-1 (ICAM-1). The results showed that miR-146a levels were lower in RA-FLSs than control fibroblasts. miR-146a mimic and Tak-242 decreased RA-FLS proliferation and increased RA-FLS apoptosis, while miR-146a inhibitor had an opposite trend. miR-1...Continue Reading

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Citations

May 18, 2020·International Immunopharmacology·Gholamreza Rezaei DanbaranHamed Mohammadi
Feb 10, 2021·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Ming ChenWei Wei
Dec 23, 2020·Thrombosis and Haemostasis·Ana B ArroyoRocío González-Conejero
Jan 25, 2020·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Wen Shi LeeTatsuya Atsumi
Apr 28, 2020·Endocrine, Metabolic & Immune Disorders Drug Targets·Xuling LuoZhiwei Chen
Dec 4, 2019·Biochemical and Biophysical Research Communications·Ioanna PapathanasiouAspasia Tsezou
Sep 16, 2020·Biochimica Et Biophysica Acta. General Subjects·Angela AvenosoGiuseppe M Campo

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Methods Mentioned

BETA
amputation
enzyme linked immunosorbent assay
ELISA
transfection
electrophoresis
flow cytometry

Software Mentioned

Primer
Pro Plus
Target Scan
SPSS18
Image J
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