MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer

Thyroid : Official Journal of the American Thyroid Association
Chunxiao YuLing Gao

Abstract

MiR-146b has been reported to be over-expressed in papillary thyroid cancer (PTC) tissues and showed association with aggressive PTC. MiR-146b-5p has been confirmed to increase cell proliferation by repressing SMAD4, however as the other expression of miR-146b, detailed functional analysis of miR-146b-3p has not been carried out. In this study, we aimed to identify miR-146b-5p and miR-146b-3p differentially expressed in PTC with more aggression, such as lymph node metastasis and further elucidate the contribution and mechanism of miR-146b-3p in the process of PTC metastasis. Expression of miR-146b-5p and miR-146b-3p was assessed in formalin-fixed paraffin-embedded (FFPE) tissues samples of PTC patients and the relationship with lymph node metastasis was analyzed.. A variety of PTC cells including BHP10-3 and K1 cells were cultured and miR-146b-5p or miR-146b-3p were over-expressed with microRNA mimics. The cell abilities of migration and invasion were detected by RTCA assay and transwell assay. PTC xenograft models were used to examine the effect of miR-146b-3p on PTC metastasis ability in vivo. Direct downstream target of miR-146b-3p was analyzed by luciferase reporter assay and western blot. The mechanism of miR-146b-3p on cell...Continue Reading

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Citations

May 19, 2020·Future Oncology·Sadegh RajabiMehdi Hedayati
Aug 8, 2021·International Journal of Molecular Sciences·Daniel Casartelli de Santa-InezEdna Teruko Kimura

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Methods Mentioned

BETA
transfection
transfections
xenograft
immunoprecipitation
Protein Assay
PCR
bioluminescence imaging
fluorescence imaging
xenografts

Software Mentioned

PASW Statistics

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