MicroRNA-150 contributes to ischemic stroke through its effect on cerebral cortical neuron survival and function by inhibiting ERK1/2 axis via Mal.

Journal of Cellular Physiology
Hui LvYu-Qin Che

Abstract

Ischemic stroke, caused by the blockage of blood supply, is a major cause of death worldwide. For identifying potential candidates, we explored the effects microRNA-150 (miR-150) has on ischemic stroke and its underlying mechanism by developing a stable middle cerebral artery occlusion (MCAO) rat model. Gene expression microarray analysis was performed to screen differentially expressed genes associated with MCAO. We evaluated the expression of miR-150 and Mal and the status of ERK1/2 axis in the brain tissues of MCAO rats. Then the cerebral cortical neurons (CCNs) were obtained and introduced with elevated or suppressed miR-150 or silenced Mal to validate regulatory mechanisms for miR-150 governing Mal in vitro. The relationship between miR-150 and Mal was verified by dual luciferase reporter gene assay. Besides, cell growth and apoptosis of CCNs were detected by means of MTT assay and flow cytometry analyses. We identified Mal as a downregulated gene in MCAO, based on the microarray data of GSE16561. MiR-150 was over-expressed and negatively targeted Mal in the brain tissues obtained from MCAO rats and their CCNs. Increasing miR-150 blocked the ERK1/2 axis, resulting in an inhibited cell growth of CNNs but an enhanced apoptos...Continue Reading

References

Jan 1, 1989·Stroke; a Journal of Cerebral Circulation·E Z LongaR Cummins
May 1, 1986·Stroke; a Journal of Cerebral Circulation·J B BedersonH Bartkowski
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Beiyan ZhouHarvey F Lodish
Jan 15, 2008·Journal of Neuroscience Research·Nik SaweHeng Zhao
Jan 27, 2009·Glia·Jonathan D VerrierLucia Notterpek
Apr 14, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Xiaoxiao Gu, Kristyn S Masters
Jul 7, 2010·Molecular Cell·Yujing ZhangChen-Yu Zhang
Nov 5, 2011·Stroke; a Journal of Cerebral Circulation·Liping LiuYongjun Wang
Jan 21, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nicholas GoplenRafeul Alam
Jun 16, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yoshihiro MurataEng H Lo
Dec 12, 2012·Medical Molecular Morphology·Samir Kumar PalTetsuhiko Tachikawa
Sep 13, 2013·Molecular Neurobiology·Mayank Chaturvedi, Leszek Kaczmarek
Nov 6, 2013·Naunyn-Schmiedeberg's Archives of Pharmacology·Lu WangHongli Shan
Mar 19, 2014·International Journal of Molecular Medicine·Jia HuangLidian Chen
Jun 21, 2014·Cancer·Mauricio BurottoElise C Kohn
Jul 15, 2015·Current Vascular Pharmacology·Ke-Jie YinY Eugene Chen
Nov 10, 2015·Scientific Reports·Norah L SmithBrian D Rudd
Nov 27, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jie ZhuYun Li
Feb 19, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhi FangBo Hu
May 18, 2016·Internal Medicine·Zhong-Bao YangXiu-Ju Luo
Jun 2, 2016·International Journal of Molecular Medicine·Gun Ho ChoiNam Keun Kim
Aug 23, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Ting-Ying Liang, Ji-Yu Lou
Nov 3, 2016·Thyroid : Official Journal of the American Thyroid Association·Shubin HongHaipeng Xiao
Nov 16, 2016·Drug Design, Development and Therapy·Tatyana A GudashevaSergey B Seredenin
Mar 4, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Michel DesjarlaisAlain Rivard
Aug 15, 2017·Progress in Neuro-psychopharmacology & Biological Psychiatry·José Javier Miguel-HidalgoDamian G Romero

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Citations

Dec 1, 2019·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Аida KondybayevaАnatoliy Ivashchenko
Jan 17, 2021·Biology of Sex Differences·Klaudia Pawlina-TyszkoMonika Bugno-Poniewierska
Nov 4, 2020·Current Pharmaceutical Design·Stavroula A PaschouDimitrios Tousoulis

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