MicroRNA-155-enhanced autophagy in human gastric epithelial cell in response to Helicobacter pylori

Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association
Kai WuJunshan Zhai

Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNAs acting as posttranscriptional gene expression regulators in many physiological and pathological conditions. MiR-155 is one kind of miRNAs that plays an important role in causing various diseases. However, the precise molecular mechanism of the ectopic expression of miR-155 in Helicobacter pylori infection remains poorly understood. Autophagy has recently been identified as an effective way to control the intracellular bacterium survival. In the present study, we demonstrate a novel role of miR-155 in regulating the autophagy-mediated anti-H. pylori response. Totally 86 H. pylori-positive patients together with 10 H. pylori-negative, healthy control subjects were included in the study. Correlation between immunohistochemical grades and miR-155 expression were determined. Molecular mechanism of miR-155 on regulation of autophagy and elimination of intracellular H. pylori were determined using the GES-1 cell model. We found that overexpression of miR-155 by transfecting miR-155 mimics could significantly decrease the survival of intracellular H. pylori, and this process was through induction of autophagy. Furthermore, there was a significant correlation between miR-155 and imm...Continue Reading

Citations

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Methods Mentioned

BETA
ELISA
protein assay
transfection
confocal microscopy

Software Mentioned

DuoSet ELISA Development System
SPSS

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