MicroRNA-155 induces autophagy in osteoclasts by targeting transforming growth factor β-activated kinase 1-binding protein 2 upon lipopolysaccharide stimulation

Bone
Ok-Joo SulHye-Seon Choi

Abstract

The autophagy pathway has been suggested to influence skeletal structure by modulating bone metabolism. Recent findings suggest that microRNAs (miR) play a critical role in autophagy. We hypothesized that inflammation induces miR-155, which enhances autophagy in osteoclasts (OC), leading to inflammatory bone loss. The expression of miR-155 was elevated in tibiae from LPS-injected mice and in OC stimulated by lipopolysaccharide (LPS) compared with vehicle treatment. Overexpression of miR-155 enhanced autophagy as well as differentiation in OC, whereas inhibition of endogenous miR-155 decreased both. Transforming growth factor β-activated kinase 1-binding protein 2 (TAB2) was identified as a target gene of miR-155 via binding to the 3'-UTR of TAB2, which directly interacts with BECLIN1. BECLIN1 was dissociated from TAB2, which started to associate with TAK1 when autophagy was induced. Our data demonstrate that LPS-induced miR-155 promoted autophagy to increase OC formation via decreased TAB2.

Citations

Aug 20, 2019·British Journal of Pharmacology·Margherita PuppoPhilippe Clézardin
Mar 23, 2019·Frontiers in Immunology·Claire LozanoFlorence Apparailly
Nov 7, 2019·Oxidative Medicine and Cellular Longevity·Hyun-Jung ParkHye-Seon Choi
May 17, 2019·Oxidative Medicine and Cellular Longevity·Ok-Joo SulHye-Seon Choi
Sep 18, 2020·Biological Research·Yang YangWang Shengwang
Oct 4, 2020·Biomolecules·Azadeh MontaseriAntonio Filippini
Oct 30, 2020·Viruses·Enakshi RoySt Patrick Reid
Jan 17, 2021·Journal of Cellular Physiology·Yi-Fan GuoXiang-Hang Luo
Feb 3, 2021·Autophagy·Soudeh Ghafouri-FardMohammad Taheri
May 24, 2021·The Journal of Nutritional Biochemistry·Ok-Joo SulHye-Seon Choi
Jul 6, 2021·Frontiers in Cell and Developmental Biology·Kazuki InoueBaohong Zhao
Jul 17, 2021·Journal of Zhejiang University. Science. B·Yuhua LiuHongchuan Jin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms