MicroRNA-155 promotes apoptosis in SKOV3, A2780, and primary cultured ovarian cancer cells

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Wei ChenYuanli He

Abstract

MicroRNAs (miRNAs) are a large group of small non-coding RNAs that can negatively regulate gene expression at the post-transcriptional level. The deregulation of miRNAs has been associated with tumorigenesis, drug resistance, and prognosis in cancers. Deregulated miR-155 has been reported in numerous cancers; however, its function remains unclear. 4',6-Diamidino-2-phenylindole (DAPI) staining and terminal-deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) techniques were used to determine the effects of a miR-155 mimic or inhibitor on the apoptotic ratio of ovarian cancer cells induced by cisplatin. Bioinformatic predictions, the dual-luciferase reporter assay, and western blot analysis were used to detect how miR-155 regulates X-linked inhibitor of apoptosis protein (XIAP). We demonstrated that a miR-155 mimic could decrease the IC50 value of cisplatin in SKOV3 ovarian cancer cells. Subsequently, gain- and loss-of-function analyses with a miR-155 mimic and inhibitor showed that miR-155 sensitizes ovarian cancer cells to cisplatin. Furthermore, the results from the luciferase assays and western blot analysis identified XIAP as the direct target of miR-155. In addition, introducing XIAP cDNA without a three prime un...Continue Reading

References

May 16, 2000·Biological Signals and Receptors·J LiB K Tsang
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 3, 2003·The Journal of Biological Chemistry·Han C DanJin Q Cheng
Mar 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Peggy S EisJames E Dahlberg
Jul 26, 2005·The Journal of Pathology·Joost KluiverAnke van den Berg
Aug 17, 2005·Cancer Research·Marilena V IorioCarlo M Croce
Mar 15, 2006·Cancer Cell·Nozomu YanaiharaCurtis C Harris
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Oct 3, 2006·Developmental Cell·Wigard P Kloosterman, Ronald H A Plasterk
Dec 7, 2006·International Journal of Cancer. Journal International Du Cancer·Eun Joo LeeThomas D Schmittgen
Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ryan M O'ConnellDavid Baltimore
Feb 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Robert W GeorgantasCurt I Civin
Aug 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Prasun J MishraJoseph R Bertino
Oct 30, 2007·Biochemical and Biophysical Research Communications·Shizuka MasakiTsukuru Umemura
Nov 22, 2007·International Journal of Cancer. Journal International Du Cancer·Tanya J ShawBarbara C Vanderhyden
Apr 17, 2008·Cancer Research·Elizabeth M SwisherToshiyasu Taniguchi
Dec 25, 2008·Cancer Biology & Therapy·Nils HabbeAnirban Maitra
Apr 8, 2010·The Journal of Biological Chemistry·William KongJin Q Cheng
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Jul 2, 2011·Nature·UNKNOWN Cancer Genome Atlas Research Network
Aug 9, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Fanfei KongGang Chen
May 23, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yiliang ZhangShuhan Sun
Apr 10, 2013·Molecular and Cellular Biochemistry·Chunmei ZhangHuayu Deng
May 30, 2013·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Wen-Jing LiuHong Shu

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Citations

Nov 2, 2018·Genes, Chromosomes & Cancer·Jean-Jacques MichailleEsmerina Tili
Nov 6, 2017·Cancer Biomarkers : Section a of Disease Markers·Jiangcheng ZuoJiancheng Tu
Jan 3, 2019·Cytotechnology·Gizem Ors-KumogluCigir Biray-Avci
Apr 4, 2021·Cancers·Shailendra Kumar Dhar DwivediResham Bhattacharya
Oct 2, 2021·Journal of Ovarian Research·Meysam Moghbeli

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