MicroRNA-155 suppresses the catabolic effect induced by TNF-α and IL-1β by targeting C/EBPβ in rat nucleus pulposus cells

Connective Tissue Research
Jie ZhouWei Ye

Abstract

miR-155 is a pro-inflammatory or anti-inflammatory factor depending on the cell type in which it is expressed. miR-155 controls apoptosis and matrix degradation in nucleus pulposus (NP) cells in vitro. The aim of this study is to explore the effect of miR-155 in vivo and further investigate the mechanism of miR-155 in vitro. MRI, hematoxylin-eosin staining, or Collagen-II immunochemistry were performed to observe intervertebral disk degeneration in conditional miR-155 overexpression mice and miR-155 knockout mice. In vitro, a dual luciferase reporter assay, real-time PCR and western blot experiments were performed to demonstrate the effect of miR-155 on the expression of catabolic genes induced by inflammatory cytokines and determine the role of β-catenin and C/EBPβ in the miR-155-mediated modulation of the expression of catabolic genes. Degeneration was observed in the lumbar disks of 1-year-old miR-155 knockout mice but not in the conditional miR-155 overexpression mice. miR-155 overexpression repressed the catabolic effect induced by TNF-α or IL-1β in vitro. Furthermore, specifically in NP cells, miR-155 overexpression suppressed the expression of C/EBPβ but not of β-catenin. Additionally, in the loss-of-function experiments...Continue Reading

References

Mar 9, 2000·Spine·K LuomaA Lamminen
Nov 27, 2004·Spine·Michele C BattiéEric Parent
Oct 3, 2006·Developmental Cell·Wigard P Kloosterman, Ronald H A Plasterk
Apr 28, 2007·Science·To-Ha ThaiKlaus Rajewsky
Feb 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maurizio CeppiPhilippe Pierre
Oct 19, 2010·Biochemical and Biophysical Research Communications·R Krishnan KuttyT Michael Redmond
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mariola Kurowska-StolarskaIain B McInnes
Feb 2, 2013·The Spine Journal : Official Journal of the North American Spine Society·Nam V VoJames D Kang
Nov 30, 2013·Arthritis Research & Therapy·Akihiko HiyamaJoji Mochida
Apr 26, 2015·Molecular and Cellular Biochemistry·Huajiang ChenWen Yuan
May 23, 2015·Experimental & Molecular Medicine·Surajit PathakIgnazio Castagliuolo
Sep 22, 2015·European Cells & Materials·Z I JohnsonM V Risbud
Oct 28, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Akihiko HiyamaJoji Mochida
Nov 21, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ming-liang JiChen Wang
Jan 26, 2016·Osteoarthritis and Cartilage·Stefania D'AdamoMartin K Lotz
Jan 28, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Quanbo JiYan Wang
May 27, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wei-Lin ZhangZhuo-Jing Luo
Oct 23, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Xiaofei WangWei Ye
Sep 17, 2016·Disease Markers·Dongping YeWeiguo Liang

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Citations

Nov 27, 2019·Spine·Srikanth N DiviChristopher K Kepler
Sep 14, 2019·International Journal of Molecular Sciences·Ehsan RazmaraSadegh Babashah
Jan 25, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Xianwei ZhouWenming Zhang
Jul 27, 2021·Frontiers in Pharmacology·Jing ZhouSimin Chen
Jun 29, 2021·Experimental and Therapeutic Medicine·Fengguang YangQingshan Yang

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