MicroRNA-181b regulates endotoxin tolerance by targeting IL-6 in macrophage RAW264.7 cells

Journal of Inflammation
Wenjun ZhangYanmei Ma

Abstract

Interleukin 6 (IL-6) is a major pro-inflammatory cytokine and dysregulation of IL-6 is relevant to many inflammatory diseases. Endotoxin induced tolerance of IL-6 is an important mechanism to avoid the excessive immune reaction. But to date, the molecular mechanisms of endotoxin tolerance of IL-6 remain unclear. Here we reported that IL-6 secretion and microRNA-181b (miR-181b) expression were inversely correlated following LPS stimulation. We also demonstrated that miR-181b targeting the 3'-UTR of IL-6 transcripts and up-regulation of miR-181b was associated with NF-kB. We further demonstrated that up-regulation of miR-181b in response to LPS was required for inducing IL-6 tolerance in macrophage. Our results suggested that the post-transcriptional control mediated by miR-181b could be involved in fine tuning the critical level of IL-6 expression in endotoxin tolerance.

References

Mar 1, 1996·Journal of Dermatological Science·Y YamakawaH Nakajima
Aug 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Q WangP O Hasselgren
Nov 16, 2002·Immunity·Ichiko KinjyoAkihiko Yoshimura
Nov 28, 2002·Diabetes·Joseph J SennRobert A Mooney
Jan 14, 2003·Annual Review of Immunology·Kiyoshi TakedaShizuo Akira
Jun 7, 2003·Drug News & Perspectives·Doris M ThamJohn C Rutledge
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Taro MutoTasuku Honjo
Mar 29, 2007·Animal Health Research Reviews·J R Stabel
Jan 22, 2008·Nature Reviews. Immunology·Harvey F LodishChang-Zheng Chen
Sep 17, 2008·The International Journal of Biochemistry & Cell Biology·Liselotte BäckdahlStephan Beck
Oct 15, 2008·The International Journal of Biochemistry & Cell Biology·Martin Hirst, Marco A Marra
Feb 18, 2010·International Journal of Cancer. Journal International Du Cancer·Wei ZhuPing Liu
Mar 12, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Feng ZhiYilin Yang
Apr 14, 2010·Scandinavian Journal of Immunology·L LiY-J Li
May 4, 2010·The Journal of Biological Chemistry·Mohamed El Gazzar, Charles E McCall
Jun 11, 2010·Annual Review of Biochemistry·Marc Robert FabianWitold Filipowicz
Mar 6, 2012·Journal of Leukocyte Biology·Edel M QuinnHenry P Redmond

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Citations

Mar 9, 2019·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Noha Ahmed El Boghdady, Olfat Gamil Shaker
Aug 23, 2020·British Medical Bulletin·Kiran RiasatMandy J Peffers
Nov 20, 2016·Science China. Life Sciences·Dongdong TiWeidong Han
Jan 17, 2019·Journal of Immunology Research·Jussara Gonçalves FernandesMarcelo De Franco
May 7, 2019·Frontiers in Immunology·Graziella CurtaleMassimo Locati
Sep 25, 2018·Journal of Cellular Biochemistry·Dan LiuYuxia Xiong
Sep 16, 2017·Frontiers in Immunology·Jezrom Bokcaerin Self-FordhamSalvador Nares
Sep 25, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Joanna M Sadowska, Maria-Pau Ginebra
Oct 30, 2020·International Journal of Molecular Sciences·Ankita SharmaNiel A Karrow

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Methods Mentioned

BETA
transfection
ELISA
PCR
transfections
immunoprecipitation
ChIP

Software Mentioned

RNA22
miRanda
SPSS

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