microRNA-18b modulates insulin-like growth factor-1 expression in deer antler cell proliferation by directly targeting its 3' untranslated region

DNA and Cell Biology
Wei HuXingyu Meng

Abstract

Insulin-like growth factor-1 (IGF-1) is a multipromoter gene that has complex biological functions and plays an important role in Chinese sika deer antler cell differentiation and proliferation. microRNAs and their roles in deer antler growth have attracted much attention. In the present study, to investigate the effect of microRNAs on the regulation of IGF-1 during the rapid growth of antlers, miRNA GeneChip analysis and TargetScan Human software were used to screen microRNAs that bind to the 3' untranslated region (3'UTR) of IGF-1. The results indicated that a significantly differential expression of miR-18b was observed in cartilage and mesenchymal of antler tip tissue and the presence of miR-18b-binding sites within the IGF-1 3'UTR. A miR-18b mimic was then transfected into antler cartilage cells to overexpress miR-18b and the expression levels were quantified by real-time PCR. Real-time PCR showed that the expression level of miR-18b in transfected cells was significantly increased compared with the control group (p<0.01). Dual luciferase assays revealed that miR-18b decreased the fluorescence value of the luciferase reporter gene in the group transfected with the wild-type vector of IGF-1 3'UTR. In contrast, the relative ...Continue Reading

References

Oct 1, 1995·Bone·S C MillerW S Jee
Apr 29, 1998·Hormone Research·G A WertherG Butler
Sep 29, 2001·Molecular Pathology : MP·Z Laron
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Sep 17, 2004·Nature·Victor Ambros
Nov 19, 2004·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Kristen E GovoniSubburaman Mohan
Mar 21, 2007·Genes & Development·Fuchou TangM Azim Surani
Sep 20, 2008·Biochemical and Biophysical Research Communications·Xi-Yong YuYangxin Li
Dec 17, 2008·Seminars in Cell & Developmental Biology·Uwe KierdorfJoanna S Price
May 27, 2010·The Journal of Biological Chemistry·Jianyin LongFarhad R Danesh
Sep 18, 2012·Biochemical and Biophysical Research Communications·Yangxin LiYong-Jian Geng
Aug 7, 2013·Biochemical and Biophysical Research Communications·Xuejuan LiuYulin Li

❮ Previous
Next ❯

Citations

Apr 10, 2016·International Journal of Biological Macromolecules·Hui TaoKai-Hu Shi
Dec 28, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Jing-Jing YangJun Li
Mar 27, 2021·Molecular Genetics and Genomics : MGG·Boyin JiaHengxing Ba

❮ Previous
Next ❯

Methods Mentioned

BETA
dissecting
chip
chips
PCR
transfection
Assay
flow cytometry
electrophoresis

Software Mentioned

Target ScanHuman
TargetScan Human
Image
Target Scan Human
SPSS
CellQuest
microRNA QC Tool
Pro Plus
TargetScan

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.