microRNA 193a-5p Regulates Levels of Nucleolar- and Spindle-Associated Protein 1 to Suppress Hepatocarcinogenesis

Gastroenterology
Sanchari RoyTom Luedde

Abstract

We performed an integrated analysis to identify microRNAs (miRNAs) and messenger RNAs (mRNAs) with altered expression in liver tumors from 3 mouse models of hepatocellular carcinoma (HCC) and human tumor tissues. We analyzed miRNA and mRNA expression profiles of liver tissues from mice with diethylnitrosamine-induced hepatocarcinogenesis, conditional expression of lymphotoxin alpha and lymphotoxin beta, or inducible expression of a Myc transgene (Tet-O-Myc mice), as well as male C57BL/6 mice (controls). miRNA mimics were expressed and miRNAs and mRNAs were knocked down in human (Huh7, Hep3B, JHH2) hepatoma cell lines; cells were analyzed for viability, proliferation, apoptosis, migration, and invasion. Cells were grown as xenograft tumors in nude mice and analyzed. We combined in silico target gene prediction with mRNA profiles from all 3 mouse models. We quantified miRNA levels in 146 fresh-frozen tissues from patients (125 HCCs, 17 matched nontumor tissues, and 4 liver samples from patients without cancer) and published human data sets and tested correlations with patient survival times using Kaplan-Meier curves and the log-rank test. Levels of NUSAP1 mRNA were quantified in 237 HCCs and 5 nontumor liver samples using the Taq...Continue Reading

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Citations

Apr 17, 2019·Journal of Cellular Physiology·Mengxuan ZhuBinbin Liu
Jul 25, 2019·Journal of Cellular Biochemistry·Wenkai ZhouQichang Yan
Jun 9, 2019·The Journal of Biological Chemistry·Joseph Y Ong, Jorge Z Torres
Feb 9, 2020·Gut·Mihnea Paul DragomirGeorge Adrian Calin
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Nov 30, 2019·Bioscience Reports·Meiyuan YangDaorong Wang
Feb 13, 2020·International Journal of Environmental Research and Public Health·Chengzhang Li, Jiucheng Xu
Apr 23, 2020·Signal Transduction and Targeted Therapy·Yuzu ZhaoHongjuan Cui
Sep 2, 2020·International Journal of Molecular Sciences·Beatrice PoliniPaola Nieri
Feb 6, 2021·International Journal of Molecular Sciences·Raphael MohrChristoph Roderburg

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