MicroRNA-195-5p, a new regulator of Fra-1, suppresses the migration and invasion of prostate cancer cells

Journal of Translational Medicine
Jian WuLiping Xie

Abstract

An increasing number of studies have demonstrated that deregulation of microRNAs (miRNAs) was a common event in tumor tissues and miRNAs would be treated as ideal tumor biomarkers or therapeutic targets. miR-195-5p (termed as miR-195 for briefly in the following part) was suggested to function as a tumor suppressor in cancer development and progression. However, the roles of miR-195 in human prostate cancer are still elusive. Thus, this study was performed to investigate the biological functions and its molecular mechanisms of miR-195 in human prostate cancer cell lines, discussing whether it has a potential to be a therapeutic way of prostate cancer. Two human prostate cancer cell lines were analyzed for the expression of miR-195 by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). A gain-of-function study of miR-195 was conducted by transfecting mimics into DU145 and PC3 cells and cell motility and invasion ability were evaluated by wound healing assay and transwell assay. Tissue microarray, and immunohistochemistry with antibodies against Fra-1 was performed using the peroxidase and DAB methods. The target gene of miR-195 was determined by luciferase assay, quantitative RT-PCR and western blot....Continue Reading

References

Jan 2, 2003·Nature Reviews. Cancer·Patricia S Steeg
Aug 17, 2005·Cancer Research·Marilena V IorioCarlo M Croce
Aug 4, 2006·Cancer Research·George Adrian Calin, Carlo Maria Croce
Nov 18, 2006·The Journal of Biological Chemistry·Gary K ScottChristopher C Benz
May 3, 2007·Genes & Development·Xiaowei ZhuMichael Snyder
Jul 10, 2007·Cancer Research·Kati P PorkkaTapio Visakorpi
Oct 16, 2007·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D L ZanetteM A Zago
Dec 22, 2007·International Journal of Cancer. Journal International Du Cancer·Chrysovalantis AndreolasAlexander Pintzas
Feb 9, 2008·The International Journal of Biochemistry & Cell Biology·Ian M ClarkDylan R Edwards
May 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Thian-Sze WongWilliam Ignace Wei
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Dec 20, 2008·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·M OsakiT Ochiya
Apr 21, 2009·International Journal of Cancer. Journal International Du Cancer·Takahiro IchimiNaohiko Seki
May 15, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Teng XuShi-Mei Zhuang
Dec 10, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Patsy Siok Hwa SoonStan B Sidhu
May 18, 2010·International Journal of Cancer. Journal International Du Cancer·Jan C BraseHolger Sültmann
Aug 24, 2010·Biochemical and Biophysical Research Communications·Lin LiuXiaohui Du
Dec 3, 2010·The Journal of Pathology·Thalia A FaraziThomas Tuschl
Feb 8, 2011·European Urology·James W F CattoTapio Visakorpi
Feb 26, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Dan LiLihui Lai
Apr 26, 2011·Pharmacology & Therapeutics·Elena Sotillo, Andrei Thomas-Tikhonenko
Jun 21, 2011·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Aug 24, 2011·Endocrine-related Cancer·Deniz M ÖzataWeng-Onn Lui
Sep 21, 2011·Genetics and Molecular Biology·Edenir Inês PalmeroMarcia Maria C Marques
Jul 18, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jea-Hyun BaekThomas Hieronymus

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Citations

Mar 30, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yawen MaYunhui Liu
Mar 27, 2018·The Journal of Gene Medicine·Olga E BryzgunovaPavel P Laktionov
Sep 8, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Cintia MassilloAdriana De Siervi
Oct 16, 2019·Molecular Biology Reports·Xiaoyan JiangSongshu Xiao
Aug 28, 2020·EMBO Reports·Geeta RaoPriyabrata Mukherjee
Mar 6, 2017·Molecular Medicine Reports·Lu JinYongqing Lai
Mar 29, 2020·Frontiers in Cell and Developmental Biology·Hong ZhanJun Lin
Mar 11, 2020·Genetics and Molecular Biology·Eliakym Arámbula-MerazNoemí García-Magallanes
Jan 3, 2020·International Journal of Molecular Medicine·Ting YuanJi-Qiang Liu
Oct 12, 2018·Molecular and Cellular Biochemistry·Finosh G ThankamDevendra K Agrawal
Feb 18, 2021·Biomedical Engineering Online·Leonardo J Galvão-LimaElio J S S Barreto

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Methods Mentioned

BETA
xenografts
transfection
PCR
Assay
protein assay
electrophoresis

Software Mentioned

TargetScan
Image J
SPSS

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