MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells

Oncology Reports
Rong GuiXin-Min Nie

Abstract

VEGF induces deterioration of hepatocellular carcinoma (HCC) by enhancing cell proliferation and migration. MicroRNAs regulate many cellular processes. In this study, we examined the regulation of tumorigenesis in HCC cells by microRNAs in relation to the effect of VEGF. Differences in microRNA expression between HepG2 and THLE-3 cells were characterized by microarray analysis. The results showed that miR-199a-5p expression was markedly downregulated in HepG2 cells and was inhibited in VEGF-overexpressing HepG2 cells in a dose- and time-dependent manner. This miRNA also inhibited cell proliferation and migration, as demonstrated by MTT and cell migration assays. Oxidored-nitro domain-containing protein 1 (NOR1), a nitroreductase, was identified as a downstream target gene of miR-199a-5p, and upregulation of NOR1 proved critical for the inhibition of VEGF-induced cell proliferation and migration in HepG2 cells by miR-199a-5p. These results indicate that miR-199a-5p is critical for regulating cell proliferation and migration by targeting and upregulating NOR1 in human HepG2 cells.

References

Sep 17, 2004·Nature·Victor Ambros
Aug 25, 2009·Translational Oncology·Armando MagrelliDomenica Taruscio
Mar 13, 2010·Methods in Molecular Biology·Michaela ScherrMatthias Eder
Sep 4, 2010·Cell Cycle·Danish Sayed, Maha Abdellatif
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Apr 12, 2012·Carcinogenesis·Marilena V Iorio, Carlo M Croce
Sep 21, 2013·British Journal of Cancer·K MiyaharaUNKNOWN Okayama Liver Cancer Group
Oct 19, 2013·Molecular Pharmacology·Sergey RyzhovIgor Feoktistov
Nov 23, 2013·Nature Reviews. Cancer·Hira Lal Goel, Arthur M Mercurio
Mar 7, 2014·Diagnostic Pathology·Zhong-xue SuCheng-kun Qin
May 17, 2014·International Journal of Molecular Sciences·Xin-E ShiGong-She Yang

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Citations

Dec 19, 2019·Cancer Metastasis Reviews·Karina Bezerra SalomãoMaría Sol Brassesco
Dec 11, 2020·BioMed Research International·Mohamed H YousefAnwar Abdelnaser

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