MicroRNA-19a and microRNA-19b promote the malignancy of clear cell renal cell carcinoma through targeting the tumor suppressor RhoB

PloS One
Shaoxi NiuXu Zhang

Abstract

Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal cell carcinoma, which shows high aggressiveness and lacks biomarkers. RhoB acts as a tumor suppressor that inhibits the progression of ccRCC. In the present study, we examined the effects of oncogenic microRNAs, miR-19a and miR-19b, on RhoB expression in ccRCC cells. The results showed that both miR-19a and miR-19b could directly target the 3'untranslated region (3'UTR) of RhoB, resulting in the reduced expression of RhoB. With RT-PCR analysis, we detected the increased expression of miR-19a and miR-19b in ccRCC tissues compared to adjacent non-tumor renal tissues. These data also demonstrated an exclusive negative correlation between miR-19a/19b and RhoB expression in ccRCC specimens and cell lines. In addition, the knockdown of RhoB or overexpression of miR-19a and miR-19b in ccRCC cells could promote cell proliferation, migration and invasion. These data demonstrate the direct roles of miR-19a and miR-19b on the repression of RhoB and its consequences on tumorigenesis, cancer cell proliferation and invasiveness. These results suggest the potential clinical impact of miR-19a and miR-19b as molecular targets for ccRCC.

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Citations

Dec 14, 2018·International Journal of Molecular Sciences·Giovanna CalabreseRosalba Parenti
Apr 25, 2019·Bioscience Reports·Yizhong PengZengwu Shao
Feb 23, 2020·Cellular Microbiology·Sudha MoorthyEric A Klein
Feb 20, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yixuan ChenYoushui Gao
Dec 21, 2018·Molecular Medicine Reports·Shuilong ZhangDongliang Wang
Apr 25, 2019·International Journal of Molecular Sciences·Cristina GrangeBenedetta Bussolati
Aug 11, 2020·Cancer Metastasis Reviews·Rui-Jie ZengEn-Min Li
Jul 30, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Caterina LiciniDaniela Marzioni
May 6, 2021·International Journal of Molecular Sciences·Alessio ArdizzoneIrene Paterniti
Sep 27, 2020·Scientific Reports·Feng WuRosemary Ann Kozar

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Methods Mentioned

BETA
GTPases
xenograft
transfections
Assay
flow cytometry
transfection
GTPase
nucleotide exchange

Software Mentioned

CellQuest Pro
TargetScan
SPSS
PicTar

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