MicroRNA-200a-3p Mediates Neuroprotection in Alzheimer-Related Deficits and Attenuates Amyloid-Beta Overproduction and Tau Hyperphosphorylation via Coregulating BACE1 and PRKACB

Frontiers in Pharmacology
Linlin WangRui Liu

Abstract

Alzheimer's disease (AD) is characterized by two landmark pathologies, the overproduction of amyloid-beta peptides (Aβ), predominated by the β-amyloid protein precursor cleaving enzyme 1 (BACE1), and hyperphosphorylation of the microtubule protein, tau, because of an imbalance in a kinase/phosphatase system that involves the activation of the protein kinase A (PKA). Current evidence indicates that brain microRNAs participate in multiple aspects of AD pathology. Here, the role and underlying molecular mechanisms of microRNA-200a-3p (miR-200a-3p) in mediating neuroprotection against AD-related deficits were investigated. The expression of miR-200a-3p was measured in the hippocampus of APP/PS1 and SAMP8 mice and in an AD cell model in vitro, as well as in blood plasma extracted from AD patients. The targets of miR-200a-3p were determined using bioinformatics and dual-luciferase assay analyses. In addition, cell apoptosis was detected using flow cytometry, and related protein levels were measured using Western blot and enzyme-linked immunosorbent assay (ELISA) techniques. miR-200a-3p was confirmed to be depressed in microarray miRNA profile analysis in vitro and in vivo, suggesting that miR-200a-3p is a potential biomarker of AD. S...Continue Reading

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Citations

Dec 6, 2019·International Journal of Molecular Sciences·Julia KonovalovaAndrii Domanskyi
Jul 18, 2020·International Journal of Molecular Sciences·Alex Cleber Improta-CariaBruno Solano de Freitas Souza
Sep 26, 2020·Frontiers in Molecular Neuroscience·Wei WeiHao Li
Apr 21, 2020·Journal of Alzheimer's Disease : JAD·Bo LiuJing-Shan Shi
Nov 3, 2020·Critical Reviews in Clinical Laboratory Sciences·Diana Navas-CarrilloEsteban Orenes-Piñero
Jan 12, 2021·Current Medical Science·Chuan HeLi-Hong Kong
Nov 13, 2020·Frontiers in Neurology·Xuewen XiaoBin Jiao
Nov 12, 2020·Antioxidants·Ana Gámez-ValeroEulàlia Martí
Apr 2, 2021·Frontiers in Aging Neuroscience·Mohammad SamadianSoudeh Ghafouri-Fard
Apr 13, 2021·Frontiers in Neuroscience·Mahta MoraghebiPegah Mousavi
Jun 3, 2021·International Journal of Molecular Sciences·Chisato KinoshitaKoji Aoyama

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Methods Mentioned

BETA
PCR
Assay
electrophoresis
Flow Cytometry
ELISA
transgenic
transfection

Software Mentioned

Excel
GraphPad Prism
TargetScan

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