MicroRNA-200a-3p suppresses tumor proliferation and induces apoptosis by targeting SPAG9 in renal cell carcinoma

Biochemical and Biophysical Research Communications
Xinsheng WangXinquan Gu

Abstract

Sperm-associated antigen 9(SPAG9), as a well-recognized oncogene protein, has a critical effect on renal cell carcinoma (RCC) progression. Our study tried to explore the mediator of miR-200a-3p, a tumor suppressing miRNA on SPAG9 expression and renal cell proliferation and apoptosis. We found the expression of miR-200a-3p was significantly lower in RCC specimens. Based on in vitro assays, we found miR-200a-3p significantly inhibit cancer cell proliferation by inducing apoptosis. In addition, our study uncovered that miR-200a-3p directly regulates oncogenic SPAG9 in 786-O and ACHN cells. Silencing of SPAG9 resulted in significantly decreased in the growth and the cell cycle of the renal cancer cell lines. Understanding of oncogenic SPAG9 regulated by miR-200a-3p might be beneficial to reveal new therapeutic targets for RCC.

References

Nov 5, 1997·Experimental Cell Research·M ImotoI B Weinstein
Mar 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Manoj GargAnil Suri
Mar 19, 2013·Journal of Clinical Bioinformatics·Rishabh KalaTrygve O Tollefsbol
Apr 11, 2013·Drugs·Bernard EscudierGuru Sonpavde
Aug 27, 2013·Seminars in Oncology·Alain RavaudJoaquim Bellmunt
Nov 7, 2013·Pharmacogenomics·Francisca DiasRui Medeiros
Mar 7, 2014·Seminars in Interventional Radiology·Carole A RidgeDavid C Madoff
Apr 18, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hui LiPing He
Jan 27, 2015·European Urology·Borje LjungbergAxel Bex
Mar 31, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ruijing LuZhendong Yu
Jul 28, 2015·International Journal of Molecular Sciences·Goda G Muralidhar, Maria V Barbolina

❮ Previous
Next ❯

Citations

Oct 30, 2016·Biochemical and Biophysical Research Communications·Zheng XuJianghua Shao
Jun 27, 2019·International Journal of Immunopathology and Pharmacology·Shuquan WeiZiwen Zhao
Jul 19, 2017·Molecular and Cellular Biochemistry·Hao FuChi Zhang
Jun 6, 2018·Nature Reviews. Urology·Sophie C JoostenManon van Engeland
May 20, 2021·Biopreservation and Biobanking·Samaneh FarajiRouhollah Fathi
Jun 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun PanLijun Mao

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis