MicroRNA-200a Regulates the Development of Mandibular Condylar Cartilage

Journal of Dental Research
M UmedaIchiro Takahashi

Abstract

Mandibular condylar cartilage (MCC) is classified as secondary cartilage, the histologic structure of which is unique from that of primary cartilage. MicroRNA (miRNA) is a small noncoding RNA that binds to the messenger RNA (mRNA) target to repress its translation and plays an important role in cell differentiation, proliferation, and death. Microarray analysis revealed that miR-200a was characteristically expressed during embryonic development. We hypothesized that miR-200a may be involved in regulating the formation of cartilage during MCC growth. We investigated the function of miR-200a by transfecting an inhibitor or mimic into MCC organ and cell cultures. A histologic examination revealed the localized inhibitory effects of the miR-200a mimic and widespread enhancing effects of the inhibitor on chondrocytic differentiation in the MCC organ culture system. An immunohistochemical examination and gene expression analysis demonstrated that the miR-200a inhibitor enhanced chondrogenesis, while the mimic had the opposite effect by enhancing cell proliferation. Quantitative reverse transcription polymerase chain reaction analysis revealed that miR-200a downregulated the gene expression of chondrocyte markers. Moreover, transfecti...Continue Reading

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Citations

Dec 25, 2015·Nature Reviews. Rheumatology·Rita VicenteChristian Jorgensen
Jul 13, 2016·Current Rheumatology Reports·Gregory R Sondag, Tariq M Haqqi
Apr 29, 2018·Journal of Periodontal Research·C WangZ Cao
Apr 24, 2017·Cellular and Molecular Life Sciences : CMLS·Andrea LolliRoberta Piva
Aug 21, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Chuan WangZhengguo Cao

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Methods Mentioned

BETA
transfection
Feature Extraction
Assay

Software Mentioned

miRbase
R
Diana target
Agilent Feature Extraction

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