MicroRNA-203 Induces Apoptosis by Targeting Bmi-1 in YD-38 Oral Cancer Cells

Anticancer Research
Jae-Sung KimDo Kyung Kim

Abstract

MicroRNAs (miRNAs) are closely associated with a number of cellular processes, including cell development, differentiation, proliferation, carcinogenesis, and apoptosis. The aim of the present study was to elucidate the molecular mechanisms underlying the tumor suppressor activity of miRNA-203 (miR-203) in YD-38 human oral cancer cells. Polymerase chain reaction analysis, MTT assay, DNA fragmentation assay, fluorescence-activated cell-sorting analysis, gene array, immunoblotting, and luciferase assay were carried out in YD-38 cells. miR-203 expression was significantly down-regulated in YD-38 cells compared to expression levels in normal human oral keratinocytes. miR-203 decreased the viability of YD-38 cells in a time- and dose-dependent manner. In addition, over-expression of miR-203 significantly increased not only DNA segmentation, but also the apoptotic population of YD-38 cells. These results indicate that miR-203 overexpression induces apoptosis in YD-38 cells. Target gene array analysis revealed that the expression of the polycomb complex protein gene Bmi-1, a representative oncogene, was significantly down-regulated by miR-203 in YD-38 cells. Moreover, both mRNA and protein levels of Bmi-1 were significantly reduced in...Continue Reading

Citations

Oct 4, 2019·Oncology Letters·Chuan Fang, Yadong Li
May 28, 2020·International Journal of Molecular Sciences·Takashi TakeuchiSilvia Zappavigna
Sep 23, 2018·Journal of Experimental & Clinical Cancer Research : CR·Baojin WangJunming Yue
Nov 8, 2020·Head & Neck·Juliana CarronGustavo J Lourenço
Nov 19, 2020·Heliyon·Soudeh Ghafouri-FardZeinab Shirvani Farsani
Dec 17, 2020·Cancers·Tomasz M GrzywaPaweł K Włodarski
May 28, 2019·Cancer Letters·Ying LiuJianxun Wang
Apr 4, 2021·International Journal of Molecular Sciences·Kumar RishabhAjaikumar B Kunnumakkara

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