microRNA-203 suppresses invasion and epithelial-mesenchymal transition induction via targeting NUAK1 in head and neck cancer

Oncotarget
Mariko ObayashiTakashi Takata

Abstract

Head and neck squamous cell carcinoma (HNSCC) has a high capacity for invasion. To identify microRNAs (miRNAs) that regulate HNSCC invasion, we compared miRNA expression profiles between a parent HNSCC cell line and a highly invasive clone. The miR-200 family and miR-203 were downregulated in the clone. Here we focused on the role of miR-203 in invasion and epithelial-mesenchymal transition (EMT) induction in HNSCC. miR-203 was downregulated during EMT induction. Moreover, ectopic overexpression of miR-203 suppressed the invasion and induced mesenchymal-epithelial transition (MET) in HNSCC cells. Interestingly, we identified NUAK family SNF1-like kinase 1 (NUAK1) as a novel target gene of miR-203 by cyclopedic analysis using anti-Ago2 antibody. Increased expression of NUAK1 was observed during EMT induction, and ectopic expression of miR-203 delayed EMT induction by suppressing NUAK1 expression. Moreover, NUAK1 overexpression promoted the invasion of HNSCC cells. Importantly, NUAK1 expression was well correlated with poor differentiation, invasiveness, and lymph node metastasis in HNSCC cases. Overall, miR-203 has a tumor-suppressing role in invasion and EMT induction by targeting NUAK1 in HNSCC, suggesting miR-203 as a potenti...Continue Reading

References

Jan 5, 2002·The New England Journal of Medicine·A ForastiereD Sidransky
Nov 1, 2002·The Journal of Biological Chemistry·Atsushi SuzukiHiroyasu Esumi
Feb 26, 2004·The American Journal of Pathology·Gen-ichi KusakaiHiroyasu Esumi
Apr 3, 2004·Molecular and Cellular Biology·Atsushi SuzukiHiroyasu Esumi
Apr 20, 2004·Cancer Cell·Li MaoVassiliki A Papadimitrakopoulou
Aug 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yasusei KudoTakashi Takata
Mar 15, 2006·Cancer Cell·Nozomu YanaiharaCurtis C Harris
Aug 22, 2006·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Yasusei KudoTakashi Takata
Nov 6, 2007·Clinical & Experimental Metastasis·Geert BerxJonathan P Sleeman
Feb 5, 2008·The Journal of Thoracic and Cardiovascular Surgery·Andrew FeberVirginia R Litle
May 17, 2008·Cell Death and Differentiation·A M LenaE Candi
Jan 29, 2009·Genes & Development·Hong SuXiaozhong Wang
Sep 19, 2009·Nature Reviews. Genetics·Carlo M Croce
Nov 26, 2009·Nature Cell Biology·Ulrich WellnerThomas Brabletz
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Aug 14, 2010·EMBO Reports·Simone Brabletz, Thomas Brabletz
Dec 17, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sharanjot SainiRajvir Dahiya
Mar 26, 2011·Science·Christine L Chaffer, Robert A Weinberg
Nov 23, 2011·Journal of Cancer Research and Clinical Oncology·Xin-Zhong ChangXu-Chen Cao
Sep 1, 2012·Experimental Cell Research·Yan Wang, Yongfeng Shang
Jan 29, 2013·International Journal of Cancer. Journal International Du Cancer·Yi QuXi-Song Ke
Jun 26, 2013·Cancer Letters·Wael M Elshamy, Roy J Duhé
Jul 23, 2013·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xiangyu ZhuHong Shi
Nov 10, 2013·Nature Medicine·Wai Leong Tam, Robert A Weinberg

❮ Previous
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Citations

Aug 19, 2018·Head & Neck·Cátia Sofia da Costa DominguesMarília de Assunção Rodrigues Ferreira Dourado
Nov 7, 2017·Oncogene·T MonteverdeD J Murphy
Mar 9, 2017·Cancer Biomarkers : Section a of Disease Markers·Peng GaoYunhui Wang
Feb 1, 2017·Current Opinion in Otolaryngology & Head and Neck Surgery·Julia PaczkowskaMalgorzata Wierzbicka
Aug 21, 2019·Journal of Experimental & Clinical Cancer Research : CR·Yingying JiangWantao Chen
Feb 1, 2017·Targeted Oncology·Tamsin RobbCherie Blenkiron
May 28, 2020·International Journal of Molecular Sciences·Takashi TakeuchiSilvia Zappavigna
Dec 26, 2016·Archives of Dermatological Research·Olivera StojadinovicMarjana Tomic-Canic
Aug 20, 2018·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Jiaoyang LiuLihua Xu
May 15, 2018·International Journal of Molecular Sciences·Samadarani B S M SiriwardenaYasusei Kudo
Nov 8, 2020·Head & Neck·Juliana CarronGustavo J Lourenço
Mar 4, 2018·Cancer Discovery·Jennifer PortDaniel J Murphy
Dec 29, 2020·Journal of Medicinal Chemistry·Muhammad FaisalSo Ha Lee
Oct 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kai ShiWeihai Peng

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Methods Mentioned

BETA
PCR
transfection
chip
immunoprecipitation
Assay
protein
electrophoresis
flow cytometry
antisense oligonucleotides

Software Mentioned

Target Scan
Ago2
Target
RQ Manger
SDS
Target Scan Human

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