Abstract
Obesity due to excessive lipid accumulation is closely associated with metabolic diseases such as type 2 diabetes, insulin resistance and inflammation. Therefore, a detailed understanding of the molecular mechanisms that underlie adipogenesis is crucial to develop treatments for diseases related to obesity. Here, we found that the microRNA-204-5p (miR-204-5p) was expressed at low levels in fat tissues from obese mice fed long-term with a high-fat diet (HFD). Overexpression or inhibition of miR-204-5p in vitro in 3T3-L1 preadipocytes significantly inhibited or promoted 3T3-L1 proliferation, respectively, an effect mediated by regulating cell proliferation factors. miR-204-5p also induced preadipocyte apoptosis by directly targeting the 3' UTR region of Bcl-2, reducing the constitutive suppression of Bcl-2 on p53-dependent apoptosis. Interestingly, overexpression of miR-204-5p during adipocyte differentiation significantly increased the number of oil red O+ cells, triglyceride accumulation and the expression of markers associated with adipocyte differentiation. In contrast, inhibition of miR-204-5p had the opposite effect on 3T3-L1 adipocyte differentiation. Luciferase activity assays and qRT-PCR showed that miR-204-5p regulates ...Continue Reading
Citations
Nov 10, 2018·PloS One·Na LiQun-Li Yu
Apr 16, 2019·Current Opinion in Lipidology·Leslie R SedgemanKasey C Vickers
Feb 25, 2020·Cell Cycle·Bin JiangShujuan Zhu
Mar 10, 2020·IUBMB Life·Lin ZhangZi-Yang Shen
May 10, 2020·Journal of Cellular Physiology·Xiaomin ZhouGongshe Yang
Mar 7, 2020·BMC Medical Genetics·Xiaojie LiYan Wang
May 6, 2020·International Journal of Molecular Sciences·Jing ZhangQinghua Nie
Sep 3, 2020·Medicine·Ning ChenBiaofang Wei
Jun 6, 2020·Biology of Reproduction·Ping ZhongWenchang Zhang
Jan 5, 2019·Clinical Science·Silvia Lorente-CebriánJ Alfredo Martínez
Nov 16, 2020·Life Sciences·Wylly Ramsés García-Niño, Cecilia Zazueta
Feb 4, 2021·Journal of Animal Science and Biotechnology·Que ZhangWeijun Pang
Feb 23, 2021·Molecular Therapy. Nucleic Acids·Lisheng XieWandong She
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Xiayang JinYuzhu Luo
Jun 3, 2021·International Journal of Molecular Sciences·Agata Wawrzkiewicz-JałowieckaGraça Soveral
May 18, 2021·Epigenomics·Andrée-Anne ClémentLuigi Bouchard