MicroRNA-204-5p regulates 3T3-L1 preadipocyte proliferation, apoptosis and differentiation

Gene
Jingjing DuLi Zhu

Abstract

Obesity due to excessive lipid accumulation is closely associated with metabolic diseases such as type 2 diabetes, insulin resistance and inflammation. Therefore, a detailed understanding of the molecular mechanisms that underlie adipogenesis is crucial to develop treatments for diseases related to obesity. Here, we found that the microRNA-204-5p (miR-204-5p) was expressed at low levels in fat tissues from obese mice fed long-term with a high-fat diet (HFD). Overexpression or inhibition of miR-204-5p in vitro in 3T3-L1 preadipocytes significantly inhibited or promoted 3T3-L1 proliferation, respectively, an effect mediated by regulating cell proliferation factors. miR-204-5p also induced preadipocyte apoptosis by directly targeting the 3' UTR region of Bcl-2, reducing the constitutive suppression of Bcl-2 on p53-dependent apoptosis. Interestingly, overexpression of miR-204-5p during adipocyte differentiation significantly increased the number of oil red O+ cells, triglyceride accumulation and the expression of markers associated with adipocyte differentiation. In contrast, inhibition of miR-204-5p had the opposite effect on 3T3-L1 adipocyte differentiation. Luciferase activity assays and qRT-PCR showed that miR-204-5p regulates ...Continue Reading

Citations

Apr 16, 2019·Current Opinion in Lipidology·Leslie R SedgemanKasey C Vickers
May 6, 2020·International Journal of Molecular Sciences·Jing ZhangQinghua Nie
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Xiayang JinYuzhu Luo
Jun 3, 2021·International Journal of Molecular Sciences·Agata Wawrzkiewicz-JałowieckaGraça Soveral

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