microRNA-21 promotes osteogenic differentiation of mesenchymal stem cells by the PI3K/β-catenin pathway

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Yu-Bin MengXian-Jin Yang

Abstract

Osteogenesis of mesenchymal stem cells (MSCs) is essential for bone repair. Recently, microRNAs have been proven to play an important role in the regulation of MSC differentiation, including osteogenesis. Here, the function of microRNA-21 (miR-21) in the osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUMSCs) was investigated. Briefly, the miR-21 mimics (m-miR-21) and the antisense miR-21 (as-miR-21) were transfected to hUMSCs, and the capacity of miR-21 for the osteogenic differentiation of hUMSCs was evaluated by the expression of osteogenic markers encoding alkaline phosphatase (ALP), runt-related gene-2 (RUNX-2) and osteocalcin (OCN), as well as by Alizarin red S staining. The results indicated that the overexpression of miR-21 elevated the expression level of the osteogenesis-related genes of hUMSCs. During this process, the PI3K-AKT signaling pathway activity had an increasing tendency responding to miR-21 up-regulation. This enhancement promoted the phosphorylation of GSK-3β, leading to the stabilization and high concentration accumulation of β-catenin in cytoplasm to activate the transcription of RUNX-2, and finally increased the osteogenesis of hUMSCs. This work demonstrated that miR-21 and i...Continue Reading

References

Dec 17, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Bart O Williams, Karl L Insogna
Jan 17, 2009·Nature Reviews. Genetics·Megha Ghildiyal, Phillip D Zamore
Apr 4, 2009·The Journal of Biological Chemistry·Zhaoyong LiJane B Lian
Dec 10, 2009·Molecular and Cellular Biology·Aditi MukherjeePeter Rotwein
Mar 13, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ji-guang ZhangGuang-hai Yang
Feb 18, 2011·The Journal of Biological Chemistry·Rong HuXiang-Hang Luo
Feb 1, 2012·Nature Reviews. Endocrinology·Jane B LianYing Zhang
Apr 17, 2012·Expert Opinion on Biological Therapy·Zakareya GamieEleftherios Tsiridis
Sep 13, 2012·Cytotechnology·Paulina DuyaYanjun Zhang
Oct 18, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nan YangYan Jin
Apr 17, 2013·International Immunopharmacology·Yan BaiXiming Pu
Sep 4, 2013·Pathology Oncology Research : POR·Akiko KawakitaMasahiro Umeda
Dec 12, 2013·TheScientificWorldJournal·Ambuj KumarRituraj Purohit
Feb 15, 2014·Advances in Wound Care·M Noelle Knight, Kurt D Hankenson
Mar 15, 2014·Korean Journal of Pathology·Hyoun Wook LeeMee Sook Roh
Sep 2, 2014·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·WenHao RenKe-Qian Zhi

❮ Previous
Next ❯

Citations

Nov 22, 2015·Experimental and Molecular Pathology·Annerose BerndtJohn P Sundberg
Jul 4, 2015·Stem Cells and Development·Adam NowakowskiBarbara Lukomska
Jul 25, 2015·International Journal of Oral Science·Dian JingZhi-He Zhao
Jul 19, 2016·Cell and Tissue Research·Cong HuangSiwen Jiang
Jan 26, 2018·International Journal of Molecular Sciences·Lifang HuAirong Qian
Jul 28, 2016·Stem Cells Translational Medicine·Taisuke FurutaMitsuo Ochi
Dec 12, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Linda J Sandell
Nov 30, 2018·Journal of Cellular Physiology·Leili Aghebati-MalekiMehdi Yousefi
Jul 29, 2015·Drug Development Research·Junying ChenShiwu Dong
Sep 27, 2019·Journal of Oral Rehabilitation·Hong WangFulan Wei
Nov 13, 2019·Cell Proliferation·Mengying LiFulan Wei
May 13, 2020·Journal of Periodontology·Franz Josef StraussReinhard Gruber
Aug 12, 2020·Basic & Clinical Pharmacology & Toxicology·Xiaoqin Wang, Peter Thomsen
Jan 27, 2019·JBMR Plus·Vincent Ka-Fai ChengChing-Lung Cheung
Jul 2, 2019·International Journal of Molecular Medicine·Xiaoyan YuanJie Long
Jul 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rong ZhuYa-Ping Guo
May 10, 2019·Journal of Cellular Biochemistry·Tayebe Moghaddam, Zeinab Neshati
Feb 26, 2020·Cells·Agnieszka SmieszekKrzysztof Marycz
Sep 28, 2016·Cell Biology International·Danying Chen, Zuolin Wang
Nov 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yubin MengXianjin Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.