MicroRNA-21 regulates cell proliferation and apoptosis in H₂O₂-stimulated rat spinal cord neurons

Molecular Medicine Reports
Genlong JiaoZiyong Zhang

Abstract

Oxidative stress can alter the expression level of microRNAs (miRNAs) and has a role in oxidative damage generated by reactive oxygen species (ROS). While previous studies have demonstrated that miR‑146a, miR‑21 and miR‑150 are essential for ROS production in heart disease, the role of these miRNAs in spinal cord injuries has not yet been examined. The present study focused on examining the role of miR‑146a, miR‑21 and miR‑150 during H2O2 stimulation in rat neuronal spinal cord (RN‑sc) cells. RN‑sc cells were treated with H2O2, and cells were harvested for reverse transcription quantitative polymerase chain reaction (RT‑qPCR) to detect the expression levels of miR‑146a, miR‑21 and miR‑150. The results demonstrated that miR‑146a, miR‑21 and miR‑150 expression was upregulated during H2O2 treatment. T-cell death and apoptosis were investigated using an MTT assay and flow cytometric analysis, respectively. Following miR‑21 silencing, H2O2‑induced cell death and apoptosis were reduced in RN‑sc cells, while miR‑150 silencing had no effect. Furthermore, Smad7 was identified as a direct target of miR‑21 using a Luciferase reporter assay, RT-qPCR and western blot analysis. In addition, while H2O2 downregulated Smad7 protein expression, ...Continue Reading

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Citations

Sep 28, 2017·Neural Plasticity·Young S GwakJoong Woo Leem
Aug 2, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Zhipeng CaiHuangyuan Li
Nov 9, 2017·Molecular Medicine Reports·Jian WangYan Duan
Nov 11, 2017·Journal of Cellular Biochemistry·Lin GaoZhiqiang Zhang
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Oct 19, 2021·Experimental and Therapeutic Medicine·Duoping ChenRui Lv

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