MicroRNA-21 via Dysregulation of WW Domain-Containing Protein 1 Regulate Atrial Fibrosis in Atrial Fibrillation

Heart, Lung & Circulation
Hui TaoKai-Hu Shi

Abstract

microRNAs (miRs) have been reported to regulate cell biological functions. To explore the underlying mechanism of miR-21 involvement in patients with atrial fibrosis and atrial fibrillation (AF). In total, 49 patients (24 AF, sinus rhythm 25) aged 33-68 years old, including heart valve replacement surgery and cardiac catheterisation. The pathological changes and collagen depositions was analysed by Masson's Trichrome Staining. miR-21, TGF-β1, Smad2, p-Smad2, WWP-1, collagen I and collagen III expression were analysed by Western blotting, qRT-PCR, miR one step qRT-PCR, respectively. Treatment human cardiac fibroblasts with TGF-β1, qRT-PCR and Western blotting to find changes in miR-21, Smad2 and WWP-1 levels. Transfected human cardiac fibroblasts with miR-21 mimic and miR-21 inhibitor. Finally, cell proliferation ability was assessed by the MTT assay and flow cytometry. Compared to sinus rhythm (SR) group, the collagen volume fraction was significantly increased in AF patients. The levels of the TGF-β1, collagen I and collagen III were significantly elevated in AF group. In AF patients, the expression of miR-21 was increased, while the expression of WWP-1 was decreased. Transfected cardiac fibroblasts with miR-21 mimic increased...Continue Reading

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Citations

Jan 25, 2020·International Journal of Molecular Sciences·Branislav KuraMonika Bartekova
Aug 14, 2020·International Journal of Molecular Sciences·Estefanía Lozano-VelascoHouria Daimi
Oct 5, 2020·Progress in Biophysics and Molecular Biology·Flavia Ravelli, Michela Masè
Sep 29, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Liao-Yuan WangGuang-Yu Ji
Jul 13, 2021·Heart Failure Reviews·Kun XiangChengming Fan
Sep 25, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Laura B SnyderFrancis G Spinale

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