MicroRNA-210 promotes angiogenesis in acute myocardial infarction.

Molecular Medicine Reports
Zhong-Guo FanNai-Liang Tian

Abstract

MicroRNA-210 (miRNA-210) has been reported to be associated with angiogenesis and may serve important roles in acute myocardial infarction (AMI), which remain unclear. The present study sought to evaluate the efficacy of miRNA‑210 in AMI and to examine the potential associated mechanisms. AMI models were established in Sprague‑Dawley rats. The expression of miRNA‑210 was upregulated via transfection with lentivirus‑mediated agonists and quantitative analysis was performed using the reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Immunoblotting and RT‑qPCR were separately used to detect the expression levels of hepatocyte growth factor (HGF) in heart samples, while only the protein expression level of β‑myosin heavy chain (β‑MHC) was assessed. The expression of HGF in human umbilical vein endothelial cells under hypoxic conditions was silenced by transfecting with small interfering RNA, as demonstrated by the determination of associated protein expression levels. The microvessel density (MVD) of the infarcted myocardium was selected to be the angiogenesis efficacy endpoint, which was evaluated by platelet endothelial cell adhesion molecule immunostaining. Markedly increased expression of HGF was observed ...Continue Reading

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Citations

Nov 5, 2019·Current Stem Cell Research & Therapy·L Vinod Kumar ReddyDwaipayan Sen
Dec 28, 2019·International Journal of Molecular Sciences·Germana ZaccagniniFabio Martelli
Jun 30, 2019·Oxidative Medicine and Cellular Longevity·Yinuo GuanBin Liu
Sep 18, 2021·Frontiers in Cell and Developmental Biology·Rafael Sánchez-SánchezPilar Sepúlveda

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Methods Mentioned

BETA
transfection
protein assay

Software Mentioned

Image Pro Plus
SPSS

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