MicroRNA-212 inhibits hepatocellular carcinoma cell proliferation and induces apoptosis by targeting FOXA1

OncoTargets and Therapy
Huahua TuTiancai Zeng

Abstract

MircroRNA-212 (miR-212) is proposed as a novel tumor-related miRNA and has been found to be significantly deregulated in human cancers. In this study, the miR-212 expression was found to be obviously downregulated in hepatocellular carcinoma (HCC) tissues as compared with adjacent nontumor tissues. Clinical association analysis indicated that low expression of miR-212 was prominently correlated with poor prognostic features of HCC, including high AFP level, large tumor size, high Edmondson-Steiner grading, and advanced tumor-node-metastasis tumor stage. Furthermore, the miR-212 expression was an independent prognostic marker for predicting both 5-year overall survival and disease-free survival of HCC patients. Our in vitro studies showed that upregulation of miR-212 inhibited cell proliferation and induced apoptosis in HepG2 cells. On the contrary, downregulation of miR-212 promoted cell proliferation and suppressed apoptosis in Huh7 cells. Interestingly, we found that upregulation of miR-212 decreased FOXA1 expression in HepG2 cells. Significantly, FOXA1 was identified as a direct target of miR-212 in HCC. FOXA1 was downregulated in HCC tissues as compared with noncancerous tissues. An inverse correlation between FOXA1 and miR...Continue Reading

Citations

Jan 14, 2020·Journal of Cellular and Molecular Medicine·Wenjun ChenJianni Qi
Dec 12, 2018·Journal of Cellular Physiology·Javid Sadri NahandHossein Keyvani
Jan 10, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yu Zheng WuPak Cheung Ng
Apr 13, 2017·BioMed Research International·Braira WahidMuhammad Idrees
Dec 19, 2019·Cancer Metastasis Reviews·Karina Bezerra SalomãoMaría Sol Brassesco

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Methods Mentioned

BETA
PCR
Assay
transfection
fluorescence activated cell sorting
flow cytometry

Software Mentioned

GraphPad Prism
ImageJ
MiRanda
SPSS
Cell Quest
TargetScan

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